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<p><i>Plantago ovata</i> is bestowed with wide range of restorative as well as curative properties owing to the presence of various bioactive secondary metabolites. The seed husk of the plant known as Isabgol or Psyllium, has been used in pharmaceutical and food industries. For further genetic improvement, we developed a rapid and efficient protocol for <i>Agrobacterium</i> mediated genetic transformation coupled with accelerated in vitro regeneration. The sterilized seeds as well as shoot/leaf explants were employed for culture initiation. Subsequent optimization of callogenesis, somatic embryogenesis, direct and indirect organogenesis was conducted on Murashige and Skoog’s medium supplemented with different plant growth regulators in varying concentrations. This is the first report on successful <i>Agrobacterium</i> mediated genetic transformation in <i>P. ovata.</i> The key transformation parameters including explant type, bacterial strain, method of inoculation and duration of co-cultivation were optimized. The highest transformation efficiency (62%) was achieved using <i>Agrobacterium tumefaciens</i> (syn. <i>Rhizobium radiobacter</i>) strain LBA4404. Present work demonstrates high frequency of callus induction, followed by somatic embryogenesis and multiple shoot/leaf formation and complete plant regeneration in eight weeks.</p>

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Establishment of a platform for Agrobacterium mediated genetic transformation in Plantago ovata Forssk.

  • 期刊论文

  • 发表日期：

    2026年3月12日

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  • Tanushri Sharma,
  • Sagrika Sharma,
  • Sudha Chib,
  • Sanjana Kaul,
  • Manoj Kumar Dhar

摘要

Plantago ovata is bestowed with wide range of restorative as well as curative properties owing to the presence of various bioactive secondary metabolites. The seed husk of the plant known as Isabgol or Psyllium, has been used in pharmaceutical and food industries. For further genetic improvement, we developed a rapid and efficient protocol for Agrobacterium mediated genetic transformation coupled with accelerated in vitro regeneration. The sterilized seeds as well as shoot/leaf explants were employed for culture initiation. Subsequent optimization of callogenesis, somatic embryogenesis, direct and indirect organogenesis was conducted on Murashige and Skoog’s medium supplemented with different plant growth regulators in varying concentrations. This is the first report on successful Agrobacterium mediated genetic transformation in P. ovata. The key transformation parameters including explant type, bacterial strain, method of inoculation and duration of co-cultivation were optimized. The highest transformation efficiency (62%) was achieved using Agrobacterium tumefaciens (syn. Rhizobium radiobacter) strain LBA4404. Present work demonstrates high frequency of callus induction, followed by somatic embryogenesis and multiple shoot/leaf formation and complete plant regeneration in eight weeks.