Scholarlink

<p>The spatiotemporal regulation of anthocyanin accumulation in petals is primarily controlled by MYB transcription factors across diverse plant species, yet the upstream mechanisms that pattern <i>MYB</i> expression remain poorly understood. Here, we show that the conserved miR319/<i>TCP</i> module serves as such an upstream regulator, differentially controlling two key <i>R2R3-MYB</i> genes—<i>AN2</i> and <i>AN4</i>—to generate complex pigmentation patterns in petals of petunia (<i>Petunia hybrida</i>). Overexpression of <i>MIR319</i> in three petunia lines (V26, MD, ‘Lemei’) led to reduced overall pigmentation but enhanced venation contrast. This was accompanied by downregulation of <i>AN2</i> (the master regulator of general petal color) and upregulation of <i>AN4</i> (the vein-specific activator), revealing an opposing regulatory mode that spatially redistributes anthocyanin synthesis. Additionally, miR319-targeted <i>TCP</i>s control petal epidermal cell shape independently of the canonical regulator <i>MIXTA</i>, as <i>MIR319</i>-OE plants exhibited flattened and enlarged conical cells with enhanced cuticular brightness, resulting in loss of velvety texture—a phenotype recapitulated in <i>tcp5/6/10</i> triple mutants. These findings establish the miR319/<i>TCP</i> module as a central hub that integrates pigmentation and epidermal cell morphogenesis, offering a mechanistic framework for understanding how a single conserved module can generate diverse ornamental traits through tissue-specific regulation. They also provide new targets for breeding novel petal aesthetics in ornamental plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A regulatory module for petal aesthetics: miR319/TCP-mediated control of color and texture in Petunia hybrida

  • 期刊论文

  • 发表日期：

    2026年2月27日

查看全文
  • Tianxing Yuan,
  • Yanyan Yang,
  • Xiaohan Zhang,
  • Yulong Guo

摘要

The spatiotemporal regulation of anthocyanin accumulation in petals is primarily controlled by MYB transcription factors across diverse plant species, yet the upstream mechanisms that pattern MYB expression remain poorly understood. Here, we show that the conserved miR319/TCP module serves as such an upstream regulator, differentially controlling two key R2R3-MYB genes—AN2 and AN4—to generate complex pigmentation patterns in petals of petunia (Petunia hybrida). Overexpression of MIR319 in three petunia lines (V26, MD, ‘Lemei’) led to reduced overall pigmentation but enhanced venation contrast. This was accompanied by downregulation of AN2 (the master regulator of general petal color) and upregulation of AN4 (the vein-specific activator), revealing an opposing regulatory mode that spatially redistributes anthocyanin synthesis. Additionally, miR319-targeted TCPs control petal epidermal cell shape independently of the canonical regulator MIXTA, as MIR319-OE plants exhibited flattened and enlarged conical cells with enhanced cuticular brightness, resulting in loss of velvety texture—a phenotype recapitulated in tcp5/6/10 triple mutants. These findings establish the miR319/TCP module as a central hub that integrates pigmentation and epidermal cell morphogenesis, offering a mechanistic framework for understanding how a single conserved module can generate diverse ornamental traits through tissue-specific regulation. They also provide new targets for breeding novel petal aesthetics in ornamental plants.