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<p><i>Vigna khandalensis</i> (Santapau) Raghavan et Wadhwa is an endemic and threatened legume belonging to genus <i>Vigna</i> of the family Fabaceae. The seeds are good source of proteins, carbohydrates, fibre, minerals and other dietary ingredients. The present study attempts to optimize a suitable micropropagation protocol for large-scale propagation and conservation of <i>V. khandalensis</i>. The primary cultures were established through seed germination on MS basal medium. The cotyledonary node explants of in vitro germinated seedlings were transferred to MS medium supplemented with different plant growth regulators (PGRs) such as 6-benzylaminopurine (BAP), meta-Topolin (mT), 6-furfurylaminopurine (KN), indole-3-butyric acid (IBA), and α-naphthalene acetic acid (NAA), either alone or in combinations. Singular supplementation of BAP (2.5&#xa0;mg/l) resulted with 95% shoot induction response, however inclusion of lower concentrations of auxins triggered the shoot production. Highest shoot induction frequency (100%), maximum number of shoots per explant (11.4 ± 0.8) as well as shoot length (9.3 ± 0.3&#xa0;cm) were achieved on MS medium fortified with a combination of BAP (2.5&#xa0;mg/l) and NAA (0.2&#xa0;mg/l). In vitro derived-shoots were transferred on different concentrations and combinations of IBA, NAA and indole-3-acetic acid (IAA). The best rooting response (100%) with an average of 19.8 ± 1.2 roots per plant was obtained on half-strength MS medium supplemented with IBA (1.5&#xa0;mg/l). The plantlets survived well when transferred to planting substrate containing a mixture of sterile soil, sand and vermiculate (1:1:1) during hardening and acclimatization stage. Further, a comparative foliar micro-morphometric and stem anatomical characteristics were analyzed through light microscopy and scanning electron microscopy (SEM) for determining the developmental adaptations of foliage of in vitro derived and field grown plantlets of <i>V. khandalensis</i>. The micropropagation protocol developed in this study could significantly contribute to the large-scale propagation, conservation and sustainable utilization of <i>V. khandalensis</i>.</p>

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Micropropagation and conservation of Vigna Khandalensis (Santapau) Raghavan et Wadhwa (Fabaceae): a promising legume as future crop for food security

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Minal M. Bodhe,
  • Dada D. Namdas,
  • Jaykumar J. Chavan

摘要

Vigna khandalensis (Santapau) Raghavan et Wadhwa is an endemic and threatened legume belonging to genus Vigna of the family Fabaceae. The seeds are good source of proteins, carbohydrates, fibre, minerals and other dietary ingredients. The present study attempts to optimize a suitable micropropagation protocol for large-scale propagation and conservation of V. khandalensis. The primary cultures were established through seed germination on MS basal medium. The cotyledonary node explants of in vitro germinated seedlings were transferred to MS medium supplemented with different plant growth regulators (PGRs) such as 6-benzylaminopurine (BAP), meta-Topolin (mT), 6-furfurylaminopurine (KN), indole-3-butyric acid (IBA), and α-naphthalene acetic acid (NAA), either alone or in combinations. Singular supplementation of BAP (2.5 mg/l) resulted with 95% shoot induction response, however inclusion of lower concentrations of auxins triggered the shoot production. Highest shoot induction frequency (100%), maximum number of shoots per explant (11.4 ± 0.8) as well as shoot length (9.3 ± 0.3 cm) were achieved on MS medium fortified with a combination of BAP (2.5 mg/l) and NAA (0.2 mg/l). In vitro derived-shoots were transferred on different concentrations and combinations of IBA, NAA and indole-3-acetic acid (IAA). The best rooting response (100%) with an average of 19.8 ± 1.2 roots per plant was obtained on half-strength MS medium supplemented with IBA (1.5 mg/l). The plantlets survived well when transferred to planting substrate containing a mixture of sterile soil, sand and vermiculate (1:1:1) during hardening and acclimatization stage. Further, a comparative foliar micro-morphometric and stem anatomical characteristics were analyzed through light microscopy and scanning electron microscopy (SEM) for determining the developmental adaptations of foliage of in vitro derived and field grown plantlets of V. khandalensis. The micropropagation protocol developed in this study could significantly contribute to the large-scale propagation, conservation and sustainable utilization of V. khandalensis.