Scholarlink

<p>Somatic embryogenesis (SE) represents a canonical manifestation of cell totipotency in plants. However, its application in woody species like Chinese fir (<i>Cunninghamia lanceolata</i>) is limited by callus browning and low embryogenic callus induction frequency. Here, we systematically compared non-embryogenic, embryogenic, and browning callus tissues by the integrated analysis of transcriptomic, proteomic, and targeted metabolomic data in Chinese fir. Immature embryo-derived non-embryogenic callus generated pearl-like embryogenic callus upon subculture, yet browning-induced cellular damage in both types severely impaired somatic embryogenesis. Furthermore, the phenylpropane flavonoid pathway is strongly and coordinately activated in browning callus, with key biosynthetic genes such as <i>PAL</i>, <i>4CL</i>, and <i>CHS</i> and their encoded proteins consistently up-regulated. Among 183 detected flavonoid metabolites, 23 showed differential accumulation, and 15 were specifically enriched in browning tissue. In addition, we constructed a multi-level evidence chain spanning genes, proteins and metabolites, which revealed that the enzymatic oxidation of phenolic compounds into highly reactive flavonoid derivatives (especially free aglycones), acts as the core mechanism driving callus browning and concomitantly inhibiting embryogenic conversion. The findings not only advance understanding of SE limitations in perennial woody plants but also offer a broadly applicable framework for improving tissue culture systems in other recalcitrant tree species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrated multi-omics analysis reveals flavonoid-driven mechanisms underlying callus browning in Cunninghamia lanceolata

  • 期刊论文

  • 发表日期：

    2026年2月2日

查看全文
  • Huiling Chen,
  • Zhengquan He,
  • Yuying Yang,
  • Yazhi Zhao,
  • Huan Liu,
  • Jiayue Li,
  • Minghao Li,
  • Longhua Yu,
  • Xudong Feng,
  • Renying Zhuo,
  • Yueqiao Li,
  • Xiaojiao Han

摘要

Somatic embryogenesis (SE) represents a canonical manifestation of cell totipotency in plants. However, its application in woody species like Chinese fir (Cunninghamia lanceolata) is limited by callus browning and low embryogenic callus induction frequency. Here, we systematically compared non-embryogenic, embryogenic, and browning callus tissues by the integrated analysis of transcriptomic, proteomic, and targeted metabolomic data in Chinese fir. Immature embryo-derived non-embryogenic callus generated pearl-like embryogenic callus upon subculture, yet browning-induced cellular damage in both types severely impaired somatic embryogenesis. Furthermore, the phenylpropane flavonoid pathway is strongly and coordinately activated in browning callus, with key biosynthetic genes such as PAL, 4CL, and CHS and their encoded proteins consistently up-regulated. Among 183 detected flavonoid metabolites, 23 showed differential accumulation, and 15 were specifically enriched in browning tissue. In addition, we constructed a multi-level evidence chain spanning genes, proteins and metabolites, which revealed that the enzymatic oxidation of phenolic compounds into highly reactive flavonoid derivatives (especially free aglycones), acts as the core mechanism driving callus browning and concomitantly inhibiting embryogenic conversion. The findings not only advance understanding of SE limitations in perennial woody plants but also offer a broadly applicable framework for improving tissue culture systems in other recalcitrant tree species.