Scholarlink

<p>Although differences in mineral requirements among plant species, cultivars, and developmental stages are well documented under ex vitro conditions, few studies have quantified such variations across species growing in vitro under comparable experimental and analytical conditions within a single laboratory framework, despite the use of species-specific culture protocols. Here, we examined the mineral nutrition of four species with contrasting tissue culture responses and growth habits (<i>Nicotiana tabacum</i>, <i>Selenicereus costaricensis</i>, <i>Guadua angustifolia</i>, and <i>Dendrocalamus giganteus</i>) grown on Murashige and Skoog (MS) medium. We evaluated tissue mineral nutrient accumulation, net utilization efficiency, and chemical speciation. Net utilization efficiency is used here as an integrative indicator defined as the percentage of the nutrient initially supplied in the medium that is recovered in plant tissues after one culture cycle. Mineral concentrations in plant tissues and in the culture medium were quantified by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), except for N, which was determined by dry combustion. For most nutrients, the net utilization efficiency was less than 50%, with <i>S. costaricensis</i> and <i>G. angustifolia</i> showing the lowest values (&lt; 25% and &lt; 5%, respectively). However, <i>D. giganteus</i> recovered ≥50% of N, P, and S and increased aerial dry mass at 3.52&#xa0;mg day⁻¹ within three weeks, whereas <i>N. tabacum</i> reached 4.17&#xa0;mg day⁻¹ and showed near-complete P recovery. Nutrient allocation patterns also differed among species: while <i>N. tabacum</i> and <i>S. costaricensis</i> accumulated more nutrients in aerial tissues, <i>G. angustifolia</i> stored several elements predominantly in the roots. Chemical speciation modeling of the MS medium showed that over 90% of most nutrients were initially present as dissolved, operationally bioavailable species. Nevertheless, elements predicted to be less available (e.g., Cu and Fe) were recovered in substantial amounts in the plant tissues, suggesting dynamic changes in speciation during culture. These findings show the importance of integrating tissue mineral nutrient accumulation, net utilization efficiency, and chemical speciation to better understand species-specific mineral nutrition patterns in vitro and to provide a diagnostic framework that can guide the design of future, tailored media for specific species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Nutrients uptake and chemical speciation reveal species-specific mineral nutrition patterns in four plant species in vitro

  • 期刊论文

  • 发表日期：

    2026年1月19日

查看全文
  • Andrea Holst,
  • María Viñas,
  • Juan C. Méndez,
  • Paul Solórzano-Cascante,
  • Eric Guevara,
  • Víctor M. Jiménez

摘要

Although differences in mineral requirements among plant species, cultivars, and developmental stages are well documented under ex vitro conditions, few studies have quantified such variations across species growing in vitro under comparable experimental and analytical conditions within a single laboratory framework, despite the use of species-specific culture protocols. Here, we examined the mineral nutrition of four species with contrasting tissue culture responses and growth habits (Nicotiana tabacum, Selenicereus costaricensis, Guadua angustifolia, and Dendrocalamus giganteus) grown on Murashige and Skoog (MS) medium. We evaluated tissue mineral nutrient accumulation, net utilization efficiency, and chemical speciation. Net utilization efficiency is used here as an integrative indicator defined as the percentage of the nutrient initially supplied in the medium that is recovered in plant tissues after one culture cycle. Mineral concentrations in plant tissues and in the culture medium were quantified by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), except for N, which was determined by dry combustion. For most nutrients, the net utilization efficiency was less than 50%, with S. costaricensis and G. angustifolia showing the lowest values (< 25% and < 5%, respectively). However, D. giganteus recovered ≥50% of N, P, and S and increased aerial dry mass at 3.52 mg day⁻¹ within three weeks, whereas N. tabacum reached 4.17 mg day⁻¹ and showed near-complete P recovery. Nutrient allocation patterns also differed among species: while N. tabacum and S. costaricensis accumulated more nutrients in aerial tissues, G. angustifolia stored several elements predominantly in the roots. Chemical speciation modeling of the MS medium showed that over 90% of most nutrients were initially present as dissolved, operationally bioavailable species. Nevertheless, elements predicted to be less available (e.g., Cu and Fe) were recovered in substantial amounts in the plant tissues, suggesting dynamic changes in speciation during culture. These findings show the importance of integrating tissue mineral nutrient accumulation, net utilization efficiency, and chemical speciation to better understand species-specific mineral nutrition patterns in vitro and to provide a diagnostic framework that can guide the design of future, tailored media for specific species.