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<p>Anthocyanins are water-soluble pigments that act as antioxidants to protect plants from adverse environmental stresses. However, the transcriptional networks that govern anthocyanin accumulation in tree species remain to be explored. Here, we identified three lines of <i>Populus simonii × P. nigra</i> (B1-359, B7-71, and B6-44), which accumulate significantly more anthocyanins in the barks than two other poplar lines (B1-345 and B4-268). Transcriptomic profiling identified 389 differentially expressed genes (DEGs) in the three lines, whose products were enriched in the phenylpropanoid biosynthetic pathway. The expressions of genes encoding the enzymes involved in anthocyanin biosynthesis were markedly elevated in B1-359, B7-71, and B6-44. In addition, three R2R3-MYB transcription factors <i>PsnMYB3</i>,<i> PsnMYB4</i> and <i>PsnMYB7</i> were strongly up-regulated and functionally implicated in anthocyanin accumulation in the three lines. Dual-luciferase reporter assays further demonstrated that <i>PsnMYB3 trans</i>-activated the promoters of anthocyanin biosynthetic genes, thereby promoting anthocyanin synthesis. These findings provide new insights into the molecular regulation of anthocyanin biosynthesis in poplar.</p>

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Transcriptome analysis reveals regulatory processes of transcription factors on anthocyanin accumulation in the bark of Populus simonii × P. nigra

  • 期刊论文

  • 发表日期：

    2025年11月27日

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  • Meiqi Lu,
  • Quyen Quynh Thi Lai,
  • Xinyu Wang,
  • Song Chen,
  • Siyuan Li,
  • Yuanfu Liu,
  • Zhanchen Ren,
  • Huiling Yan,
  • Qiushuang Zhao,
  • Haoran Bao,
  • Ling Jin,
  • Yunxia Wei,
  • Jiaxin Liu,
  • Su Chen

摘要

Anthocyanins are water-soluble pigments that act as antioxidants to protect plants from adverse environmental stresses. However, the transcriptional networks that govern anthocyanin accumulation in tree species remain to be explored. Here, we identified three lines of Populus simonii × P. nigra (B1-359, B7-71, and B6-44), which accumulate significantly more anthocyanins in the barks than two other poplar lines (B1-345 and B4-268). Transcriptomic profiling identified 389 differentially expressed genes (DEGs) in the three lines, whose products were enriched in the phenylpropanoid biosynthetic pathway. The expressions of genes encoding the enzymes involved in anthocyanin biosynthesis were markedly elevated in B1-359, B7-71, and B6-44. In addition, three R2R3-MYB transcription factors PsnMYB3, PsnMYB4 and PsnMYB7 were strongly up-regulated and functionally implicated in anthocyanin accumulation in the three lines. Dual-luciferase reporter assays further demonstrated that PsnMYB3 trans-activated the promoters of anthocyanin biosynthetic genes, thereby promoting anthocyanin synthesis. These findings provide new insights into the molecular regulation of anthocyanin biosynthesis in poplar.