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<p>WRKY is a family of plant-specific transcription factors and plays an important role in plant growth, development and abiotic stress. However, <i>WRKY70</i> has not been previously identified in herbaceous peony. In this study, we isolated the <i>PlWRKY70</i> gene from the WRKY family in herbaceous peony. The <i>PlWRKY70</i> coding sequence (CDS) was 933&#xa0;bp and encodes 310 amino acids. According to the analysis by SMART online tool, the secondary and tertiary structures was predominantly random coil, and the phosphorylation primarily occurs on serine. Phylogenetic analysis revealed that <i>PlWRKY70</i> protein was closely related to peony and contains a conserved WRKY domain. The quantitative real-time PCR (qRT-PCR) analysis indicated that <i>PlWRKY70</i> gene was higher expression in leaves and significant increase under salt stress. Subcellular localization revealed that PlWRKY70 protein was predominantly located in the nuclear. Overexpressed <i>PlWRKY70</i> in <i>Arabidopsis thaliana</i> revealed significantly reduced malondialdehyde (MDA) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels under salt stress compared to wild-type (WT) plants, along with increased antioxidant enzyme activity and total antioxidant capacity (T-AOC), and overexpression lines exhibited significant salinity resistance than WT. These results suggested that <i>PlWRKY70</i> enhanced the salt-tolerance in transgenic <i>A. thaliana</i> by increasing the antioxidant enzyme activity and reducing reactive oxygen species (ROS) induced membrane damage, thereby providing a basis for further investigation into the <i>PlWRKY70</i> gene’s role in herbaceous peonies and its salt stress response mechanisms.</p>

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A herbaceous peony WRKY transcription factor PlWRKY70 enhances tolerance to salt stress in transgenic Arabidopsis thaliana

  • 期刊论文

  • 发表日期：

    2025年11月19日

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  • Ling Tang,
  • Weili Long,
  • Yanhua Pan,
  • Jiaxuan Ren,
  • Weicheng Wang,
  • Rong Huang,
  • Fei Shen

摘要

WRKY is a family of plant-specific transcription factors and plays an important role in plant growth, development and abiotic stress. However, WRKY70 has not been previously identified in herbaceous peony. In this study, we isolated the PlWRKY70 gene from the WRKY family in herbaceous peony. The PlWRKY70 coding sequence (CDS) was 933 bp and encodes 310 amino acids. According to the analysis by SMART online tool, the secondary and tertiary structures was predominantly random coil, and the phosphorylation primarily occurs on serine. Phylogenetic analysis revealed that PlWRKY70 protein was closely related to peony and contains a conserved WRKY domain. The quantitative real-time PCR (qRT-PCR) analysis indicated that PlWRKY70 gene was higher expression in leaves and significant increase under salt stress. Subcellular localization revealed that PlWRKY70 protein was predominantly located in the nuclear. Overexpressed PlWRKY70 in Arabidopsis thaliana revealed significantly reduced malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels under salt stress compared to wild-type (WT) plants, along with increased antioxidant enzyme activity and total antioxidant capacity (T-AOC), and overexpression lines exhibited significant salinity resistance than WT. These results suggested that PlWRKY70 enhanced the salt-tolerance in transgenic A. thaliana by increasing the antioxidant enzyme activity and reducing reactive oxygen species (ROS) induced membrane damage, thereby providing a basis for further investigation into the PlWRKY70 gene’s role in herbaceous peonies and its salt stress response mechanisms.