<p>Weed infestation in rapeseed fields significantly impacts crop yield, making the development of herbicide-resistant rapeseed varieties and hybrids a crucial breeding objective. Early selection of resistant plants can be achieved through marker-assisted selection; however, this method only allows qualitative detection of resistance. We have developed an in vitro method based on selective media to quantitatively assess and rank plants according to their resistance level to imidazolinones. This laboratory-based approach aligns with sustainability goals, as it eliminates herbicide release into the environment and ensures human safety, while also being unaffected by weather or season. This approach can either complement marker-assisted selection or serve as an independent evaluation tool. Our study demonstrated that a concentration of 12.5&#xa0;mg/L imazamox in the medium effectively selects plants with resistance levels comparable to commercial hybrids, while a gradient of higher imazamox concentrations enables the ranking of plants by resistance strength. The ability to compare and select highly resistant plants is valuable for both breeding programs and genetic studies aimed at identifying new, more resistant mutants.</p>

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Selection of rapeseed for resistance to imidazolinone group of herbicides on solid media under in vitro conditions

  • Elena Kozar,
  • Ilya Chepovoy,
  • Maria Fomicheva,
  • Elena Domblides

摘要

Weed infestation in rapeseed fields significantly impacts crop yield, making the development of herbicide-resistant rapeseed varieties and hybrids a crucial breeding objective. Early selection of resistant plants can be achieved through marker-assisted selection; however, this method only allows qualitative detection of resistance. We have developed an in vitro method based on selective media to quantitatively assess and rank plants according to their resistance level to imidazolinones. This laboratory-based approach aligns with sustainability goals, as it eliminates herbicide release into the environment and ensures human safety, while also being unaffected by weather or season. This approach can either complement marker-assisted selection or serve as an independent evaluation tool. Our study demonstrated that a concentration of 12.5 mg/L imazamox in the medium effectively selects plants with resistance levels comparable to commercial hybrids, while a gradient of higher imazamox concentrations enables the ranking of plants by resistance strength. The ability to compare and select highly resistant plants is valuable for both breeding programs and genetic studies aimed at identifying new, more resistant mutants.