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<p>This study evaluated the genetic effects of EMS (ethyl methanesulfonate) treatments (0.1%–0.6% for 60–180&#xa0;min) on plants regenerated from callus tissues of <i>Solanum tuberosum</i> L. cv. Agria. Callus formation occurred only in the Agria cultivar; no callus was observed in Marfona and Lady Olimpia. ISSR (Inter Simple Sequence Repeat) markers were used to assess genetic variation in EMS-treated regenerants. ISSR-PCR analysis revealed that lower EMS concentrations (0.1%–0.2%) with longer durations (180&#xa0;min) preserved genetic similarity, while higher concentrations (0.6%) and shorter durations (60&#xa0;min) increased genetic distance and polymorphism. PCA and UPGMA analyses supported a strong correlation between EMS dose/duration and genetic variability. These findings demonstrate the mutagenic efficiency of EMS in callus-based regeneration and highlight the high resolution of ISSR markers for detecting induced variation. <i>The results</i> contribute to mutation-based breeding strategies aiming to enhance genetic variation in potato.</p>

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Genetic variation in EMS-treated potato calli assessed by ISSR

  • 期刊论文

  • 发表日期：

    2025年11月5日

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  • Nüket Altındal,
  • Tahsin Karadoğan,
  • Demet Altındal

摘要

This study evaluated the genetic effects of EMS (ethyl methanesulfonate) treatments (0.1%–0.6% for 60–180 min) on plants regenerated from callus tissues of Solanum tuberosum L. cv. Agria. Callus formation occurred only in the Agria cultivar; no callus was observed in Marfona and Lady Olimpia. ISSR (Inter Simple Sequence Repeat) markers were used to assess genetic variation in EMS-treated regenerants. ISSR-PCR analysis revealed that lower EMS concentrations (0.1%–0.2%) with longer durations (180 min) preserved genetic similarity, while higher concentrations (0.6%) and shorter durations (60 min) increased genetic distance and polymorphism. PCA and UPGMA analyses supported a strong correlation between EMS dose/duration and genetic variability. These findings demonstrate the mutagenic efficiency of EMS in callus-based regeneration and highlight the high resolution of ISSR markers for detecting induced variation. The results contribute to mutation-based breeding strategies aiming to enhance genetic variation in potato.