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<p>Hairy root (HR) cultures represent a valuable biotechnological platform for the production of bioactive compounds. In this study, an optimized protocol was developed for the induction and maintenance of HR cultures from the Argentine peanut (<i>Arachis hypogaea</i> L.) cultivar Granoleico, using <i>Rhizobium rhizogenes</i> strain LBA9402. Cotyledon explants exhibited higher transformation efficiency (80%) when cultivated in solid Hoagland (Hg) medium compared to the conventional half-strength Murashige and Skoog (½ MS) medium. Among the independent HR lines obtained, line C9 showed the highest growth index (GI = 21.8) and growth rate (GR = 165.9 mg day<sup>−1</sup>) in liquid Hoagland medium supplemented with vitamins (HgVit). Biochemical characterization of C9 revealed that unsaturated fatty acids accounted for 71% of total lipids, with linoleic (36.5%) and linolenic acids (23.3%) as the most abundant. The total phenolic content reached 2982.7 mg GAE g<sup>−1</sup> FW, and antioxidant activity was 2.15 mM AAE g<sup>−1</sup> FW (24% DPPH inhibition). HPLC-MS analysis identified four flavonoids, predominantly naringin (1.62 ng mg<sup>−1</sup> FW) and genistein (0.45 ng mg<sup>−1</sup> FW). These findings demonstrate that peanut HR cultures from cv. Granoleico constitutes an efficient in vitro system for the production of antioxidant- and lipid-rich metabolites with potential applications in food, pharmaceutical, and agricultural biotechnology.</p>

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Biotechnological potential of hairy roots from an argentine peanut (Arachis hypogaea L.) type runner cultivar granoleico

  • 期刊论文

  • 发表日期：

    2025年11月5日

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  • Gabriela Gazalez Vargas,
  • Mariana Elisa Vezza,
  • Marta Susana Dardanelli,
  • Ana Laura Wevar Oller,
  • Natalia Paulucci

摘要

Hairy root (HR) cultures represent a valuable biotechnological platform for the production of bioactive compounds. In this study, an optimized protocol was developed for the induction and maintenance of HR cultures from the Argentine peanut (Arachis hypogaea L.) cultivar Granoleico, using Rhizobium rhizogenes strain LBA9402. Cotyledon explants exhibited higher transformation efficiency (80%) when cultivated in solid Hoagland (Hg) medium compared to the conventional half-strength Murashige and Skoog (½ MS) medium. Among the independent HR lines obtained, line C9 showed the highest growth index (GI = 21.8) and growth rate (GR = 165.9 mg day−1) in liquid Hoagland medium supplemented with vitamins (HgVit). Biochemical characterization of C9 revealed that unsaturated fatty acids accounted for 71% of total lipids, with linoleic (36.5%) and linolenic acids (23.3%) as the most abundant. The total phenolic content reached 2982.7 mg GAE g−1 FW, and antioxidant activity was 2.15 mM AAE g−1 FW (24% DPPH inhibition). HPLC-MS analysis identified four flavonoids, predominantly naringin (1.62 ng mg−1 FW) and genistein (0.45 ng mg−1 FW). These findings demonstrate that peanut HR cultures from cv. Granoleico constitutes an efficient in vitro system for the production of antioxidant- and lipid-rich metabolites with potential applications in food, pharmaceutical, and agricultural biotechnology.