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<p><i>Agrobacterium</i>-mediated transformation remains the most effective and widely used method for introducing foreign genes into tomato, a model crop among dicotyledonous species for developmental and functional studies. Despite the availability of standardized protocols for model tomato cultivars, transforming recalcitrant non-model tomato cultivars remains as a challenge due to genotype-dependent responses to tissue culture conditions. Over the past decades, tomato transformation has undergone substantial refinement with growing emphasis on cultivar-specific optimization strategies. Furthermore, more research and efforts toward developing versatile, genotype-independent tomato transformation protocols are essential for expanding and fully utilizing the benefits of evolving genome editing tools for crop improvement in tomato. This mini-review provides insights into critical factors affecting successful tomato regeneration and transformation, and also highlights some of the strategies deployed in recently optimized protocols for overcoming these challenges.</p>

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Agrobacterium-mediated transformation for gene editing in tomato: challenges and advances

  • 期刊论文

  • 发表日期：

    2025年10月27日

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  • Kausalya Sakthivel,
  • Kumar K. K.,
  • Rajagopal Balasubramanian,
  • Vellaikumar Sampathrajan,
  • Ravichandran Veerasamy,
  • Sathiyamurthy V. A.

摘要

Agrobacterium-mediated transformation remains the most effective and widely used method for introducing foreign genes into tomato, a model crop among dicotyledonous species for developmental and functional studies. Despite the availability of standardized protocols for model tomato cultivars, transforming recalcitrant non-model tomato cultivars remains as a challenge due to genotype-dependent responses to tissue culture conditions. Over the past decades, tomato transformation has undergone substantial refinement with growing emphasis on cultivar-specific optimization strategies. Furthermore, more research and efforts toward developing versatile, genotype-independent tomato transformation protocols are essential for expanding and fully utilizing the benefits of evolving genome editing tools for crop improvement in tomato. This mini-review provides insights into critical factors affecting successful tomato regeneration and transformation, and also highlights some of the strategies deployed in recently optimized protocols for overcoming these challenges.