<p>This paper reports methods for successful micropropagation of <i>Taraxacum shikotanense</i> by using seedling explants and shoots stored under slow-growth conditions in combination with abscisic acid treatment. Explants were isolated from several-day-old seedlings and transferred onto MS medium supplemented with 0.25 mg·L^− 1 6-benzylaminopurine and 0.025 mg·L^− 1 naphthyl-1-acetic acid. The highest response of almost 81% frequency of shoot organogenesis with 7.6 shoots/explant was obtained from root explants. The highest rooting frequency (92%) was recorded in hydroponic culture (ex vitro method). However, 2.1 roots per shoot on 1/2 MS medium with or without NAA was the highest number of roots obtained. The results of regrowth after slow-growth culture indicated that <i>T. shikotanense</i> shoots can be stored for a period of 12 months at a temperature of 4&#xa0;°C without any significant reduction in survival or the ability of shoots to organogenesis regardless of the storage variant used (reduced temperature only or in combination with either ABA preculture or 1 mg·L^− 1 ABA treatment during storage). However, the multiplication rate significantly decreased under storage condition. The 1 mg·L^− 1 ABA preculture had slight protection against cold stress, although it resulted in a 100% shoot frequency and 6.2 shoots/explant. However, only ABA treatment during storage increased the levels of proline and ABA in the cold-stored tissue. Exogenous ABA treatment had no effect on the level of JA. Moreover, it was found that both conditions during regeneration and cold storage had no effect on the flowering process and seed viability after acclimatization to field conditions.</p>