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<p>This study aimed to optimize pepper anther culture by assessing genotypic responses and the efficacy of antimicrobial agents. A significant genotypic influence on culture success was observed across fourteen F1 hybrids, with embryo induction and seedling formation rates ranging from 0.107% to 3.438% and 0.013% to 2.220%, respectively. Among the tested genotypes, B23-5 demonstrated superior performance, exhibiting the highest efficiency for both parameters. While several agents, including beta-aminobutyric acid (BABA), plant preservative mixture (PPM), and penicillin, significantly reduce contamination, they also exhibited significant phytotoxicity. In stark contrast, 50 mM gamma-aminobutyric acid (GABA) demonstrated a notably effective dual function. GABA not only controlled contamination and tissue browning but also significantly enhanced the embryo induction rate, identifying it as a highly promising supplement for future protocol. We introduce a novel approach that utilize GABA to simultaneously address the key bottlenecks of contamination and low embryogenesis in pepper anther culture. The proposed protocol offers an powerful tool to improve the efficiency of generating homozygous lines and accelerating pepper breeding programs.</p>

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Optimization of anther culture in pepper (Capsicum annuum L.) via genotype selection and GABA supplementation

  • 期刊论文

  • 发表日期：

    2025年10月21日

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  • Sihao Hou,
  • Xuefeng Gong,
  • Yi Xu,
  • Hong Li,
  • Xin Chen,
  • Zhanfeng Song,
  • Qingwei Li

摘要

This study aimed to optimize pepper anther culture by assessing genotypic responses and the efficacy of antimicrobial agents. A significant genotypic influence on culture success was observed across fourteen F1 hybrids, with embryo induction and seedling formation rates ranging from 0.107% to 3.438% and 0.013% to 2.220%, respectively. Among the tested genotypes, B23-5 demonstrated superior performance, exhibiting the highest efficiency for both parameters. While several agents, including beta-aminobutyric acid (BABA), plant preservative mixture (PPM), and penicillin, significantly reduce contamination, they also exhibited significant phytotoxicity. In stark contrast, 50 mM gamma-aminobutyric acid (GABA) demonstrated a notably effective dual function. GABA not only controlled contamination and tissue browning but also significantly enhanced the embryo induction rate, identifying it as a highly promising supplement for future protocol. We introduce a novel approach that utilize GABA to simultaneously address the key bottlenecks of contamination and low embryogenesis in pepper anther culture. The proposed protocol offers an powerful tool to improve the efficiency of generating homozygous lines and accelerating pepper breeding programs.