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<p><i>Populus davidiana × P. bolleana</i> cv. Baicheng-1 is a female hybrid distinguished by seed production without fluff, exhibits hypoallergenic traits, rendering it valuable for mass propagation. Regeneration of the poplar under the influence of thidiazuron (TDZ) and <i>meta</i>-Topolin (<i>m</i>-T) at concentrations of 0.1, 0.5, and 1 µM on Murashige and Skoog (MS) medium from leaves and single-node segments of aseptic shoots was first studied. Direct organogenesis was observed with all types and concentrations of phytohormones. Nodal segments produced both axillary and adventitious shoots, while leaf explants generated only adventitious shoots under TDZ treatment. The number of shoots increased with higher TDZ concentrations, yielding 13.25 ± 3.0 to 26.16 ± 4.1 shoots per explant (3.4 to 4.4 times higher than <i>m</i>-T), but shoot height decreased from 12.54 ± 4.8&#xa0;mm to ≥ 5&#xa0;mm. <i>m</i>-T treatments produced more robust, and greener shoots with larger leaf blade areas. Rooting of all shoots reached 100% on hormone-free MS medium. Regenerants exhibited acclimatization survival rates of 84.7% − 100%. Preliminary assessment of clonal fidelity, using RAPD and ISSR markers, revealed an average genetic polymorphism of 58.4% between regenerants and the mother plant, and 34.4% among regenerants. The lowest variation observed in <i>m</i>-T-derived regenerants. Thus, TDZ proved the most effective for micropropagating, but <i>m</i>-T produces more viable and clonal fidelity regenerants. Nodal segments were the optimal explant type. An efficient system was developed for multiplication of <i>P. davidiana × P. bolleana</i> cv. Baicheng-1, which are hypoallergenic, ornamental, promising for urban landscaping. However, further studies of the genetic fidelity of the regenerants is necessary.</p>

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Effect of thidiazuron and meta-topolin on in vitro shoot multiplication and development of the Populus davidiana × P. bolleana hybrid

  • 期刊论文

  • 发表日期：

    2025年10月14日

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  • Tatiana Zheleznichenko,
  • Anatoly Karakulov,
  • Sergey Asbaganov,
  • Alex Kochetov

摘要

Populus davidiana × P. bolleana cv. Baicheng-1 is a female hybrid distinguished by seed production without fluff, exhibits hypoallergenic traits, rendering it valuable for mass propagation. Regeneration of the poplar under the influence of thidiazuron (TDZ) and meta-Topolin (m-T) at concentrations of 0.1, 0.5, and 1 µM on Murashige and Skoog (MS) medium from leaves and single-node segments of aseptic shoots was first studied. Direct organogenesis was observed with all types and concentrations of phytohormones. Nodal segments produced both axillary and adventitious shoots, while leaf explants generated only adventitious shoots under TDZ treatment. The number of shoots increased with higher TDZ concentrations, yielding 13.25 ± 3.0 to 26.16 ± 4.1 shoots per explant (3.4 to 4.4 times higher than m-T), but shoot height decreased from 12.54 ± 4.8 mm to ≥ 5 mm. m-T treatments produced more robust, and greener shoots with larger leaf blade areas. Rooting of all shoots reached 100% on hormone-free MS medium. Regenerants exhibited acclimatization survival rates of 84.7% − 100%. Preliminary assessment of clonal fidelity, using RAPD and ISSR markers, revealed an average genetic polymorphism of 58.4% between regenerants and the mother plant, and 34.4% among regenerants. The lowest variation observed in m-T-derived regenerants. Thus, TDZ proved the most effective for micropropagating, but m-T produces more viable and clonal fidelity regenerants. Nodal segments were the optimal explant type. An efficient system was developed for multiplication of P. davidiana × P. bolleana cv. Baicheng-1, which are hypoallergenic, ornamental, promising for urban landscaping. However, further studies of the genetic fidelity of the regenerants is necessary.