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<p>Biotechnological techniques such as micropropagation are valuable tools for the propagation and conservation of tree species. This study aimed to establish optimal conditions for in vitro seed germination and micropropagation of <i>Gallesia integrifolia</i> and to evaluate alterations in polyamine (PA) levels during shoot development. Seeds were germinated at 55% confluence in MS culture medium, whereas no germination occurred in WPM, and mechanical scarification was unnecessary. Compared with the control, supplementation with 0.5 µM 6-benzyladenine (BA) significantly increased shoot elongation, increasing shoot length by 127% in shoots from apical and 183% in shoots from cotyledonary nodal segments. This response was associated with higher endogenous free putrescine (up to + 87%) and reduced free spermidine levels (up to – 80%) in shoots treated with BA than in the control, indicating a PA-mediated role in shoot growth. Ex vitro rooting was not influenced by indole-3-butyric acid treatment; however, it was significantly affected by explant type, with shoots from cotyledonary nodal segments showing higher rooting induction than those from apical nodal segments. All rooted plantlets (100%) survived during greenhouse acclimatization. These findings not only provide a reproducible protocol for the conservation of <i>G. integrifolia</i> but also offer a practical strategy applicable to the propagation of other native Atlantic Forest species with potential use in reforestation and ecological restoration.</p>

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In vitro germination and micropropagation of Gallesia integrifolia (Spreng.) Harms (Phytolaccaceae): effects of 6-benzyladenine on shoot development and polyamine metabolism

  • 期刊论文

  • 发表日期：

    2025年9月17日

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  • Mateus Santana Rodrigues,
  • Jessica de Abreu Cruz,
  • Victor Paulo Mesquita Aragão,
  • Tadeu dos Reis de Oliveira,
  • Vanildo Silveira,
  • Claudete Santa-Catarina

摘要

Biotechnological techniques such as micropropagation are valuable tools for the propagation and conservation of tree species. This study aimed to establish optimal conditions for in vitro seed germination and micropropagation of Gallesia integrifolia and to evaluate alterations in polyamine (PA) levels during shoot development. Seeds were germinated at 55% confluence in MS culture medium, whereas no germination occurred in WPM, and mechanical scarification was unnecessary. Compared with the control, supplementation with 0.5 µM 6-benzyladenine (BA) significantly increased shoot elongation, increasing shoot length by 127% in shoots from apical and 183% in shoots from cotyledonary nodal segments. This response was associated with higher endogenous free putrescine (up to + 87%) and reduced free spermidine levels (up to – 80%) in shoots treated with BA than in the control, indicating a PA-mediated role in shoot growth. Ex vitro rooting was not influenced by indole-3-butyric acid treatment; however, it was significantly affected by explant type, with shoots from cotyledonary nodal segments showing higher rooting induction than those from apical nodal segments. All rooted plantlets (100%) survived during greenhouse acclimatization. These findings not only provide a reproducible protocol for the conservation of G. integrifolia but also offer a practical strategy applicable to the propagation of other native Atlantic Forest species with potential use in reforestation and ecological restoration.