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<p>To address the industrial bottlenecks of <i>Sinocrassula indica</i> (Decne.) Berger, including prolonged propagation cycles, poor genetic stability, and low acclimatization survival rates, this study established an efficient <i>in vitro</i> rapid propagation system. An L<sub>9</sub> (3<sup>4</sup>) orthogonal design was employed to optimize the hormonal combinations of 6-BA (0.1–1.0&#xa0;mg/L), NAA (0.05–0.5&#xa0;mg/L), and KT (0.5–2.0&#xa0;mg/L), integrated with paclobutrazol (PP333)-mediated rejuvenation culture to enhance plantlet quality. Results demonstrated that the optimal hormonal combination (0.1&#xa0;mg/L 6-BA + 0.3&#xa0;mg/L NAA + 1.0&#xa0;mg/L KT) achieved a proliferation coefficient of 93.5 (<i>P</i> &lt; 0.05). Furthermore, 0.01&#xa0;mg/L PP333 treatment Significantly improved acclimatization survival rates from 54.83 to 100%, increased stem diameter by 48% (<i>P</i> &lt; 0.01), and preserved the characteristic rosette morphology. Long-term cultivation observations have shown that the regenerated plants subjected to rejuvenation treatment maintained morphological characteristics consistent with the mother plant (such as rosette configuration, leaf waxy layer, etc.) within 10 months, with no heritable phenotypic variations observed. The established protocol provides a robust solution for germplasm conservation and industrial-scale propagation of Crassulaceae plants, offering dual benefits for ecological preservation and commercial horticultural applications.</p>

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Synergistic regulation of plant growth regulators on somatic embryogenesis and optimization of rejuvenation culture in Sinocrassula indica (Decne.) Berger

  • 期刊论文

  • 发表日期：

    2025年9月23日

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  • Li-qing Cheng,
  • Shuang Liu,
  • Heng-yu Huang,
  • Ai-li Zhang

摘要

To address the industrial bottlenecks of Sinocrassula indica (Decne.) Berger, including prolonged propagation cycles, poor genetic stability, and low acclimatization survival rates, this study established an efficient in vitro rapid propagation system. An L9 (34) orthogonal design was employed to optimize the hormonal combinations of 6-BA (0.1–1.0 mg/L), NAA (0.05–0.5 mg/L), and KT (0.5–2.0 mg/L), integrated with paclobutrazol (PP333)-mediated rejuvenation culture to enhance plantlet quality. Results demonstrated that the optimal hormonal combination (0.1 mg/L 6-BA + 0.3 mg/L NAA + 1.0 mg/L KT) achieved a proliferation coefficient of 93.5 (P < 0.05). Furthermore, 0.01 mg/L PP333 treatment Significantly improved acclimatization survival rates from 54.83 to 100%, increased stem diameter by 48% (P < 0.01), and preserved the characteristic rosette morphology. Long-term cultivation observations have shown that the regenerated plants subjected to rejuvenation treatment maintained morphological characteristics consistent with the mother plant (such as rosette configuration, leaf waxy layer, etc.) within 10 months, with no heritable phenotypic variations observed. The established protocol provides a robust solution for germplasm conservation and industrial-scale propagation of Crassulaceae plants, offering dual benefits for ecological preservation and commercial horticultural applications.