Grona styracifolia is a herbaceous plant with significant medicinal value, containing a variety of bioactive components, especially flavonoids. ‘Guangyaoda No. 1’ is a new variety of Grona styracifolia. Its upright growth characteristic is conducive to field harvesting and reduces the area of agricultural land. This study aims to explore the role of the GsC3H30 gene in the biosynthesis of flavonoids and lignin in Grona styracifolia and analyze its influence on the unique plant shape of ‘Guangyaoda No. 1’ Grona styracifolia. In this study, bioinformatics tools were utilized to conduct sequence and structural analysis of GsC3H30. The expression pattern of GsC3H30 in Grona styracifolia was analyzed by real-time fluorescence quantitative PCR (RT-qPCR) technology. The GsC3H30 gene was transferred into Arabidopsis thaliana for functional verification through cloning and vector construction. In addition, subcellular localization experiments and transcriptional activity analyses were conducted, as well as the analysis of the expression and total content of flavonoid and lignin biosynthesis genes in transgenic Arabidopsis thaliana. It was found that GsC3H30 had tissue-specific expression patterns in different varieties of Grona styracifolia. The GsC3H30 protein is mainly located in the cell nucleus and has transcriptional inhibitory activity. The phenotypes of the stems and leaves of transgenic Arabidopsis thaliana are different from those of wild Arabidopsis thaliana. The expression levels of key genes for flavonoid biosynthesis in transgenic Arabidopsis thaliana increased significantly, while the expression levels of genes related to lignin synthesis decreased. The total flavonoid content of transgenic Arabidopsis thaliana was significantly higher than that of the wild type, while the lignin content was relatively lower. It was revealed that the GsC3H30 gene affects the accumulation of secondary metabolites by regulating the expression of lignin and flavonoid synthesis genes, leading to changes in the phenotype of Arabidopsis thaliana. It provides a new perspective for understanding the molecular mechanism of the unique plant shape of ‘Guangyaoda No. 1’ Grona styracifolia and offers a theoretical basis for breeding new varieties with excellent growth characteristics and high contents of bioactive components.

Functional analysis of the differentially expressed gene GsC3H30 in Grona styracifolia

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发表日期：

2025年11月3日

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Huang Jinheng,
Zhang Jiayan,
Huang Xi,
Zhou Xinyu,
Liao Peiran,
Yang Quan

摘要

Grona styracifolia is a herbaceous plant with significant medicinal value, containing a variety of bioactive components, especially flavonoids. ‘Guangyaoda No. 1’ is a new variety of Grona styracifolia. Its upright growth characteristic is conducive to field harvesting and reduces the area of agricultural land. This study aims to explore the role of the GsC3H30 gene in the biosynthesis of flavonoids and lignin in Grona styracifolia and analyze its influence on the unique plant shape of ‘Guangyaoda No. 1’ Grona styracifolia. In this study, bioinformatics tools were utilized to conduct sequence and structural analysis of GsC3H30. The expression pattern of GsC3H30 in Grona styracifolia was analyzed by real-time fluorescence quantitative PCR (RT-qPCR) technology. The GsC3H30 gene was transferred into Arabidopsis thaliana for functional verification through cloning and vector construction. In addition, subcellular localization experiments and transcriptional activity analyses were conducted, as well as the analysis of the expression and total content of flavonoid and lignin biosynthesis genes in transgenic Arabidopsis thaliana. It was found that GsC3H30 had tissue-specific expression patterns in different varieties of Grona styracifolia. The GsC3H30 protein is mainly located in the cell nucleus and has transcriptional inhibitory activity. The phenotypes of the stems and leaves of transgenic Arabidopsis thaliana are different from those of wild Arabidopsis thaliana. The expression levels of key genes for flavonoid biosynthesis in transgenic Arabidopsis thaliana increased significantly, while the expression levels of genes related to lignin synthesis decreased. The total flavonoid content of transgenic Arabidopsis thaliana was significantly higher than that of the wild type, while the lignin content was relatively lower. It was revealed that the GsC3H30 gene affects the accumulation of secondary metabolites by regulating the expression of lignin and flavonoid synthesis genes, leading to changes in the phenotype of Arabidopsis thaliana. It provides a new perspective for understanding the molecular mechanism of the unique plant shape of ‘Guangyaoda No. 1’ Grona styracifolia and offers a theoretical basis for breeding new varieties with excellent growth characteristics and high contents of bioactive components.