Elicitation using jasmonates, salicylic acid, and sodium chloride in cell suspension cultures of Justicia gendarussa Burm f.: a route for enhanced and sustainable production of apigenin and apigetrin

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发表日期：

2025年10月15日

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Vinaya Chandran,
Maya Rajan,
S. Shahena,
Linu Mathew

摘要

This study realized a high and sustained yield of apigenin and apigetrin in Justicia gendarussa cell suspension cultures by eliciting with methyl jasmonate (MeJA), jasmonic acid (JA), salicylic acid (SA), and sodium chloride (NaCl). The friable calli induced by culturing leaf segments cultured on MS basal medium supplemented with 2 mg L⁻¹ of 2, 4-Dichlorophenoxyacetic acid (2,4-D) and 0.2 mg L⁻¹ of Benzyl Adenine (BA), was transferred to liquid MS medium containing 1 mg L⁻¹ 2, 4-D, and elicited with varying doses of elicitors on the fortieth day. Cultures treated with 200 µM MeJA recorded the highest accumulation of apigenin (123.98 ± 1.33 µg g⁻¹ DW) and apigetrin (342.87 ± 2.82 µg g⁻¹ DW) by the second day of elicitation. Also, both compounds showed an increase upon JA elicitation, with the maximum accumulation (apigetrin 114.68 ± 8.47 µg g⁻¹ DW; apigenin 73.93 ± 6.32 µg g¹ DW) at 100 µM JA by the sixth day. Likewise, SA at 100 µM boosted compound production (apigenin 106.57 ± 4.37 µg g⁻¹ DW and apigetrin 231.43 ± 7.21 µg g⁻¹ DW) on day 4. Salt stress promoted the accumulation of apigenin (107.13 ± 7.44 µg g⁻¹ DW) and apigetrin (245.31 ± 12.50 µg g⁻¹ DW), with the highest accumulation at 200 mM of NaCl. The per-day elicitation efficiency of these four elicitors was MeJA > SA > NaCl > JA. All elicitors produced higher concentrations of compounds than in leaf extracts of field-grown plants (29.69 ± 6.97 µg g⁻¹ DW of apigenin and 122.42 ± 9.83 µg g⁻¹ DW of apigetrin).