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<p>An efficient in vitro propagation method for <i>Galium asperifolium</i> Wall. was developed using nodal explants. Initially, explants were cultured on agar-gelled, Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP). Of the tested concentrations, 6&#xa0;µM BAP was optimal for the cultures. Different cytokinins viz<i>.,</i> Kinetin (KIN), meta-Topolin (mT), 6- (γ,γ-Dimethylallyl amino) purine (2-iP), and Thidiazuron (TDZ), were compared with BAP and established the efficiency of 6&#xa0;µM BAP to achieve a high rate of multiplication (13.9 shoots/explant) and explant response (100%). However, the microshoots in 6&#xa0;µM BAP were intractable due to their height, and also, the shoots raised were thin and slender, which affected the subsequent rooting and hardening. A gibberellin inhibitor, ancymidol (ANC), was tested at different concentrations (2.5, 5, 7.5, 10, and 12&#xa0;µM) along with 6&#xa0;µM BAP. A combination of ANC (10&#xa0;µM) with 6&#xa0;µM BAP produced compact and robust microshoots with maximum regeneration potential (shoot number = 65.62 shoots per explant). Microshoots raised in ANC supplemented medium treated with 10&#xa0;mM IBA for 3&#xa0;min, followed by planting in soil conditions, produced maximum rooting (85.71%) compared to the control. The inter-simple sequence repeat (ISSR) marker analysis confirmed monomorphism among all the tested plantlets. ANC in combination with BAP is an effective way to produce short and sturdy microshoots, coupled with a 1.64-fold increase in shoot proliferation compared to the control. To our knowledge, this is the first report on the micropropagation and the homogeneity assessment after micropropagation of <i>G. asperifolium</i>.</p>

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A robust method modulated by Ancymidol for the direct regeneration of Galium asperifolium Wall.

  • 期刊论文

  • 发表日期：

    2025年8月4日

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  • Sruthy S. Nair,
  • E. A. Siril

摘要

An efficient in vitro propagation method for Galium asperifolium Wall. was developed using nodal explants. Initially, explants were cultured on agar-gelled, Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP). Of the tested concentrations, 6 µM BAP was optimal for the cultures. Different cytokinins viz., Kinetin (KIN), meta-Topolin (mT), 6- (γ,γ-Dimethylallyl amino) purine (2-iP), and Thidiazuron (TDZ), were compared with BAP and established the efficiency of 6 µM BAP to achieve a high rate of multiplication (13.9 shoots/explant) and explant response (100%). However, the microshoots in 6 µM BAP were intractable due to their height, and also, the shoots raised were thin and slender, which affected the subsequent rooting and hardening. A gibberellin inhibitor, ancymidol (ANC), was tested at different concentrations (2.5, 5, 7.5, 10, and 12 µM) along with 6 µM BAP. A combination of ANC (10 µM) with 6 µM BAP produced compact and robust microshoots with maximum regeneration potential (shoot number = 65.62 shoots per explant). Microshoots raised in ANC supplemented medium treated with 10 mM IBA for 3 min, followed by planting in soil conditions, produced maximum rooting (85.71%) compared to the control. The inter-simple sequence repeat (ISSR) marker analysis confirmed monomorphism among all the tested plantlets. ANC in combination with BAP is an effective way to produce short and sturdy microshoots, coupled with a 1.64-fold increase in shoot proliferation compared to the control. To our knowledge, this is the first report on the micropropagation and the homogeneity assessment after micropropagation of G. asperifolium.