Scholarlink

<p>An outstanding feature of plants is their pronounced developmental plasticity and regenerative ability from differentiated tissues. In this study, we investigated the influence of carbon nanotubes, including single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs), on the capacity for de novo root organogenesis (rhizogenesis) and shoot organogenesis (caulogenesis) using different poplar explants. Our results showed that the application of 200 mg·L<sup>−1</sup> carbon nanotubes significantly promoted adventitious root formation at the base of stem cuttings, increasing root length by 48% (SWCNT<sub>200</sub>) and 46% (MWCNT<sub>200</sub>), primarily due to simultaneous increase in auxin and cytokinin levels as well as the upregulation of reactive oxygen species (ROS). In the shoots of poplar plantlets, 50 mg·L<sup>−1</sup> carbon nanotubes enhanced shoot fresh weight by 45% (SWCNT<sub>50</sub>) and 50% (MWCNT<sub>50</sub>), with underlying mechanisms involving enhanced photosystem II light energy utilization efficiency and activation ROS defense and antioxidant enzyme system. Carbon nanotubes in the media also significantly altered the timing and frequency of de novo shoot and root formation by modulating the biosynthesis and antagonism of auxin and cytokinin. Specifically, 50&#xa0;mg·L⁻¹ carbon nanotubes accelerated shoot organogenesis by 15%, while 200&#xa0;mg·L⁻¹ enhanced root organogenesis by 38%, with no significant difference observed between SWCNTs and MWCNTs. Carbon nanotubes-mediated regulation of organogenesis was characterized by differential expression of key meristem-identity genes, including shoot-specific <i>WUS</i> and root-specific <i>WOX5</i>. Overall, this work demonstrates the distinct effects of carbon nanotubes on the regenerative capacity of plant cells and suggests their potential as growth regulators to improve plant propagation and genetic engineering.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dose-dependent modulation of micropropagation and organogenesis in poplar tissue culture by carbon nanotubes

  • 期刊论文

  • 发表日期：

    2025年8月11日

查看全文
  • Bing Zhang,
  • Sainan Zhang,
  • Haoyu Dong,
  • Shaohui Yang,
  • Jiehua Wang

摘要

An outstanding feature of plants is their pronounced developmental plasticity and regenerative ability from differentiated tissues. In this study, we investigated the influence of carbon nanotubes, including single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs), on the capacity for de novo root organogenesis (rhizogenesis) and shoot organogenesis (caulogenesis) using different poplar explants. Our results showed that the application of 200 mg·L−1 carbon nanotubes significantly promoted adventitious root formation at the base of stem cuttings, increasing root length by 48% (SWCNT200) and 46% (MWCNT200), primarily due to simultaneous increase in auxin and cytokinin levels as well as the upregulation of reactive oxygen species (ROS). In the shoots of poplar plantlets, 50 mg·L−1 carbon nanotubes enhanced shoot fresh weight by 45% (SWCNT50) and 50% (MWCNT50), with underlying mechanisms involving enhanced photosystem II light energy utilization efficiency and activation ROS defense and antioxidant enzyme system. Carbon nanotubes in the media also significantly altered the timing and frequency of de novo shoot and root formation by modulating the biosynthesis and antagonism of auxin and cytokinin. Specifically, 50 mg·L⁻¹ carbon nanotubes accelerated shoot organogenesis by 15%, while 200 mg·L⁻¹ enhanced root organogenesis by 38%, with no significant difference observed between SWCNTs and MWCNTs. Carbon nanotubes-mediated regulation of organogenesis was characterized by differential expression of key meristem-identity genes, including shoot-specific WUS and root-specific WOX5. Overall, this work demonstrates the distinct effects of carbon nanotubes on the regenerative capacity of plant cells and suggests their potential as growth regulators to improve plant propagation and genetic engineering.