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<p>This study investigated the role of the <i>BpMYB86</i> transcription factor in enhancing salt tolerance in <i>Betula platyphylla</i> (white birch). Transgenic lines were generated by construction of vectors harboring the <i>BpMYB86-OE</i> (overexpression) and <i>BpMYB86-SE</i> (suppresssion) genes and transforming of zygotic embryos <i>via Agrobacterium tumefaciens</i>-mediated techniques, yielding 10 overexpression and 9 repression lines. Under NaCl stress, the overexpression lines exhibited significantly enhanced growth compared to the wild-type (WT) and suppression lines, exhibiting a 1.2–1.7-fold increase in fresh/dry weight, improved chlorophyll retention, and greater root biomass. Physiological analyses revealed that overexpression lines maintained lower Na<sup>+</sup>/K<sup>+</sup> ratios (15% Na<sup>+</sup> content vs. &gt;70% in SE) and elevated K<sup>+</sup> accumulation (&gt; 60% of total ions). Antioxidant capacity was also significantly elevated in overexpression lines, showing 1.4–2.5-fold higher SOD/POD activity and 36–37% lower MDA levels in comparison to WT, as evidenced by reduced H<sub>2</sub>O<sub>2</sub>/O<sup>2−</sup> accumulation in histochemical staining. Furthermore, salt-responsive genes (<i>LTP4</i>, <i>TCP10</i>, <i>SPL9</i>, <i>ERF1.1</i>, <i>bHLH112</i>) were significant upregulated in overexpression lines. Notably, no growth trade-offs were observed in unstressed overexpression lines. These results demonstrate that <i>BpMYB86</i> overexpression enhances salt tolerance by modulating ion homeostasis, boosting antioxidant activity, and activating stress-responsive gene networks, providing a genetic foundation for breeding salt-resistant birch.</p>

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Overexpression of BpMYB86 enhances salt tolerane in Betula platyphylla

  • 期刊论文

  • 发表日期：

    2025年11月6日

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  • Xi Chen,
  • Baopeng Jia,
  • Tingting Jin,
  • Zhuolong Li,
  • Meng Yang,
  • Yuzhi Zhang,
  • Jiabei Zhang,
  • Jingli Yang,
  • Haijiao Huang

摘要

This study investigated the role of the BpMYB86 transcription factor in enhancing salt tolerance in Betula platyphylla (white birch). Transgenic lines were generated by construction of vectors harboring the BpMYB86-OE (overexpression) and BpMYB86-SE (suppresssion) genes and transforming of zygotic embryos via Agrobacterium tumefaciens-mediated techniques, yielding 10 overexpression and 9 repression lines. Under NaCl stress, the overexpression lines exhibited significantly enhanced growth compared to the wild-type (WT) and suppression lines, exhibiting a 1.2–1.7-fold increase in fresh/dry weight, improved chlorophyll retention, and greater root biomass. Physiological analyses revealed that overexpression lines maintained lower Na+/K+ ratios (15% Na+ content vs. >70% in SE) and elevated K+ accumulation (> 60% of total ions). Antioxidant capacity was also significantly elevated in overexpression lines, showing 1.4–2.5-fold higher SOD/POD activity and 36–37% lower MDA levels in comparison to WT, as evidenced by reduced H2O2/O2− accumulation in histochemical staining. Furthermore, salt-responsive genes (LTP4, TCP10, SPL9, ERF1.1, bHLH112) were significant upregulated in overexpression lines. Notably, no growth trade-offs were observed in unstressed overexpression lines. These results demonstrate that BpMYB86 overexpression enhances salt tolerance by modulating ion homeostasis, boosting antioxidant activity, and activating stress-responsive gene networks, providing a genetic foundation for breeding salt-resistant birch.