Scholarlink

<p>The Ethylene-Responsive Factor 2 (ERF2) plays a critical regulatory role in multiple physiological processes associated with apple development and maturation. Based on the analysis of the apple <i>MdERF2</i> gene and the fundamental structural features and characteristics of its encoded amino acids, this study constructed an <i>MdERF2</i>-overexpressing vector using the pRI101 plasmid backbone. The construct was stably introduced into <i>Malus domestica</i> calli via <i>Agrobacterium</i>-mediated transformation. Subsequently, transcriptome sequencing was employed to investigate the effects of <i>MdERF2</i> overexpression on differentially expressed genes across metabolic pathways. The results revealed that MdERF2 modulates enzymatic browning by regulating <i>MdPAL1</i> expression, mediates tissue resistance by influencing <i>MdC4H</i> expression, participates in lignin biosynthesis through the regulation of <i>MdHST</i>, <i>MdCOMT1</i>, and <i>MdCCoAOMT5</i>, contributes to anthocyanin accumulation by regulating <i>MdGT5</i> and <i>MdANS</i>, facilitates pectin degradation via modulation of <i>MdPME</i>, delays carbohydrate metabolism in fruit through the regulation of <i>MdRPI2</i>, <i>MdPFK</i>, <i>MdPGAM</i>, and <i>MdPEPCK</i>, and is implicated in auxin and biotin metabolism by controlling the expression of <i>MdAMI1</i> and <i>MdDTBS</i>/<i>Md7</i>,<i>8-DAPAAT</i>. These findings provide critical reference value for further exploration and characterization of MdERF2 in apple fruit quality formation and maintenance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Elucidating the role of MdERF2 in apple fruit quality formation through transcriptome profiling

  • 期刊论文

  • 发表日期：

    2025年10月13日

查看全文
  • Yanfang Zhang,
  • Sijia Hou,
  • Xintong Wei,
  • Xinhua Zhang,
  • Xiaoan Li,
  • Fujun Li

摘要

The Ethylene-Responsive Factor 2 (ERF2) plays a critical regulatory role in multiple physiological processes associated with apple development and maturation. Based on the analysis of the apple MdERF2 gene and the fundamental structural features and characteristics of its encoded amino acids, this study constructed an MdERF2-overexpressing vector using the pRI101 plasmid backbone. The construct was stably introduced into Malus domestica calli via Agrobacterium-mediated transformation. Subsequently, transcriptome sequencing was employed to investigate the effects of MdERF2 overexpression on differentially expressed genes across metabolic pathways. The results revealed that MdERF2 modulates enzymatic browning by regulating MdPAL1 expression, mediates tissue resistance by influencing MdC4H expression, participates in lignin biosynthesis through the regulation of MdHST, MdCOMT1, and MdCCoAOMT5, contributes to anthocyanin accumulation by regulating MdGT5 and MdANS, facilitates pectin degradation via modulation of MdPME, delays carbohydrate metabolism in fruit through the regulation of MdRPI2, MdPFK, MdPGAM, and MdPEPCK, and is implicated in auxin and biotin metabolism by controlling the expression of MdAMI1 and MdDTBS/Md7,8-DAPAAT. These findings provide critical reference value for further exploration and characterization of MdERF2 in apple fruit quality formation and maintenance.