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<p>Species of the <i>Peperomia</i> genus are widely used as ornamental plants and exhibit herbicidal, pharmaceutical, and medicinal potential due to the presence of bioactive compounds with anticancer, antifungal, and antimicrobial properties. However, only 15% of phytochemical studies on the <i>Piperaceae</i> family involve <i>Peperomia</i>, and many species remain underexplored due to their small size and limited natural populations. This study sought to achieve in vitro propagation via shoot multiplication and organogenesis of <i>Peperomia stroemfeltii</i>, a native and endemic species to the Atlantic Forest, and to analyze the genetic stability of the plantlets. MS medium supplemented with 2.5&#xa0;mg L⁻¹ of BAP was the most efficient for shoot induction and multiplication from nodal segments, whereas for organogenesis BAP and NAA effectively promoted the development of shoots and roots from leaves. Regenerated plants from both pathways showed somaclonal variation at the DNA level using SSR markers but were phenotypically normal. Organogenesis allowed for a higher number of in vitro plants, producing between 20 and 30 plantlets. The acclimatization protocol using vermiculite combined with plant soil and Tricho-Turbo<sup>®</sup> resulted in 100% survival of regenerated plants from both pathways. This study presents an efficient protocol for the in vitro propagation of <i>P. stroemfeltii</i> from nodal and leaf segments, with the organogenic pathway being the most suitable for large-scale propagation of the species.</p>

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In vitro propagation and genetic analysis of Peperomia stroemfeltii: insights for large-scale cultivation of an endemic Atlantic forest species

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Aline dos Santos Bergamin,
  • Mayla Bessa Scotá,
  • Loren Cristina Vasconcelos,
  • Elias Terra Werner,
  • Gustavo Fernandes Mariano,
  • Ana Luiza Assis Semonato,
  • Geisiele Silva Martins,
  • Vinicius Sartori Fioresi,
  • Paulo Cezar Cavatte,
  • Marcia Flores da Silva Ferreira,
  • Milene Miranda Praça-Fontes

摘要

Species of the Peperomia genus are widely used as ornamental plants and exhibit herbicidal, pharmaceutical, and medicinal potential due to the presence of bioactive compounds with anticancer, antifungal, and antimicrobial properties. However, only 15% of phytochemical studies on the Piperaceae family involve Peperomia, and many species remain underexplored due to their small size and limited natural populations. This study sought to achieve in vitro propagation via shoot multiplication and organogenesis of Peperomia stroemfeltii, a native and endemic species to the Atlantic Forest, and to analyze the genetic stability of the plantlets. MS medium supplemented with 2.5 mg L⁻¹ of BAP was the most efficient for shoot induction and multiplication from nodal segments, whereas for organogenesis BAP and NAA effectively promoted the development of shoots and roots from leaves. Regenerated plants from both pathways showed somaclonal variation at the DNA level using SSR markers but were phenotypically normal. Organogenesis allowed for a higher number of in vitro plants, producing between 20 and 30 plantlets. The acclimatization protocol using vermiculite combined with plant soil and Tricho-Turbo® resulted in 100% survival of regenerated plants from both pathways. This study presents an efficient protocol for the in vitro propagation of P. stroemfeltii from nodal and leaf segments, with the organogenic pathway being the most suitable for large-scale propagation of the species.