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<p><i>Cattleya schilleriana</i>, an orchid species endemic to Brazil, faces an imminent threat of extinction due to habitat loss and the intensive exploitation of its natural populations. This article explores strategies for the micropropagation of <i>C. schilleriana</i> under slow growth conditions, comparing concentrations of Murashige and Skoog (MS) medium with different osmolyte concentrations, for conservation purposes and germplasm availability. The addition of osmolytes was investigated in MS and ½ MS culture media, individually and combined with three osmolytes: sucrose, mannitol, and sorbitol. Sucrose increased leaf number, aerial part length, and photosynthetic activity. Hydrogen peroxide levels increased, as did the activity of the antioxidant enzymes catalase (CAT) and ascorbate peroxidase (APX). Superoxide dismutase (SOD) activity varied along with malondialdehyde content. Mannitol and sorbitol in MS medium, similar to sucrose in ½ MS medium, enhanced rhizogenesis. Additionally, the membrane stability index was affected by in vitro maintenance and growth stress. During acclimatization, osmolyte activity remained slightly higher than that of the control plant, allowing for greater ex vitro survival. The results suggest that <i>C. schilleriana</i> exhibits a significant adaptive response to conservation. As conclusions, 2% mannitol in MS medium and 3% mannitol in MS medium were the most effective for the growth and survival of the species.&#xa0;</p> Graphical abstract <p></p>

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Adaptive response to conservation of Cattleya schilleriana Rchb.f. under slow growth conditions

  • 期刊论文

  • 发表日期：

    2025年7月24日

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  • Gracielle Vidal Silva Andrade,
  • Michele Carla Nadal,
  • Moacir Pasqual,
  • Michele Valquíria dos Reis,
  • Joyce Dória

摘要

Cattleya schilleriana, an orchid species endemic to Brazil, faces an imminent threat of extinction due to habitat loss and the intensive exploitation of its natural populations. This article explores strategies for the micropropagation of C. schilleriana under slow growth conditions, comparing concentrations of Murashige and Skoog (MS) medium with different osmolyte concentrations, for conservation purposes and germplasm availability. The addition of osmolytes was investigated in MS and ½ MS culture media, individually and combined with three osmolytes: sucrose, mannitol, and sorbitol. Sucrose increased leaf number, aerial part length, and photosynthetic activity. Hydrogen peroxide levels increased, as did the activity of the antioxidant enzymes catalase (CAT) and ascorbate peroxidase (APX). Superoxide dismutase (SOD) activity varied along with malondialdehyde content. Mannitol and sorbitol in MS medium, similar to sucrose in ½ MS medium, enhanced rhizogenesis. Additionally, the membrane stability index was affected by in vitro maintenance and growth stress. During acclimatization, osmolyte activity remained slightly higher than that of the control plant, allowing for greater ex vitro survival. The results suggest that C. schilleriana exhibits a significant adaptive response to conservation. As conclusions, 2% mannitol in MS medium and 3% mannitol in MS medium were the most effective for the growth and survival of the species. 

Graphical abstract