Scholarlink

<p>Arbutin, a hydroquinone β-D-glucopyranoside, is a high-value phytochemical known for its skin-whitening, antioxidant, and antimicrobial properties. The biotransformation of precursors such as 4-hydroxybenzoic acid (4-HBAc) and hydroquinone (HQ) to arbutin using plant-based systems offers an eco-friendly and sustainable alternative to chemical synthesis. This study explores the biotransformation potential of <i>Mesosphaerum suaveolens</i> (L.) Kuntze. and <i>Ocimum basilicum</i> L. in both solid and suspension cultures, supplemented with plant growth regulators (PGRs) including α-naphthaleneacetic acid (NAA), 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro cultures of <i>M. suaveolens</i> and <i>O. basilicum</i> were established under controlled conditions, and then 4-HBAc and HQ were administered as precursors at varying concentrations and dosing regimens. Arbutin accumulation was quantitatively analyzed using a diode array detector- high-performance liquid chromatography (DAD-HPLC). The maximum arbutin accumulation was observed in suspension cultures for both plant species. For <i>M. suaveolens</i>, optimal arbutin yield was achieved with a single dose of 288&#xa0;mg/mL 4-HBAc, while <i>O. basilicum</i> exhibited the highest arbutin accumulation with three doses of 288&#xa0;mg/mL 4-HBAc. The differential responses observed in <i>M. suaveolens</i> and <i>O. basilicum</i> highlight the importance of culture conditions and precursor management in optimizing biotransformation efficiency. Future research should focus on scaling up the biotransformation process for industrial applications, elucidating the underlying metabolic and genetic pathways involved in arbutin biosynthesis, and evaluating the economic feasibility of large-scale production. Additionally, the integration of advanced biotechnological approaches may further enhance arbutin yield and process efficiency.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unraveling the mechanism of biotransformation in Mesosphaerum suaveolens (L.) Kuntze. and Ocimum basilicum L. solid and suspension cultures using 4-hydroxybenzoic acid and hydroquinone as precursors

  • 期刊论文

  • 发表日期：

    2025年6月10日

查看全文
  • M. Riddhi,
  • A. Deepak,
  • P. Parth,
  • K. Monisha

摘要

Arbutin, a hydroquinone β-D-glucopyranoside, is a high-value phytochemical known for its skin-whitening, antioxidant, and antimicrobial properties. The biotransformation of precursors such as 4-hydroxybenzoic acid (4-HBAc) and hydroquinone (HQ) to arbutin using plant-based systems offers an eco-friendly and sustainable alternative to chemical synthesis. This study explores the biotransformation potential of Mesosphaerum suaveolens (L.) Kuntze. and Ocimum basilicum L. in both solid and suspension cultures, supplemented with plant growth regulators (PGRs) including α-naphthaleneacetic acid (NAA), 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro cultures of M. suaveolens and O. basilicum were established under controlled conditions, and then 4-HBAc and HQ were administered as precursors at varying concentrations and dosing regimens. Arbutin accumulation was quantitatively analyzed using a diode array detector- high-performance liquid chromatography (DAD-HPLC). The maximum arbutin accumulation was observed in suspension cultures for both plant species. For M. suaveolens, optimal arbutin yield was achieved with a single dose of 288 mg/mL 4-HBAc, while O. basilicum exhibited the highest arbutin accumulation with three doses of 288 mg/mL 4-HBAc. The differential responses observed in M. suaveolens and O. basilicum highlight the importance of culture conditions and precursor management in optimizing biotransformation efficiency. Future research should focus on scaling up the biotransformation process for industrial applications, elucidating the underlying metabolic and genetic pathways involved in arbutin biosynthesis, and evaluating the economic feasibility of large-scale production. Additionally, the integration of advanced biotechnological approaches may further enhance arbutin yield and process efficiency.

Graphical Abstract