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<p><i>Spathiphyllum wallisii</i>, a widely admired ornamental plant, is renowned for its beauty and air-purifying abilities, making it a prominent candidate for tissue culture propagation. Although significant progress has been made in <i>Spathiphyllum</i> micropropagation, the potential of protocorm-like bodies (PLBs) for both propagation and mid-term conservation remains underexplored. In this study, we investigated the effects of varying concentrations of MS medium and sucrose on PLB preservation using slow-growth storage over a 12-month period. Additionally, we optimized PLB proliferation by testing different concentrations of silver nitrate (AgNO₃) and adenine sulphate (ADS). Our findings revealed that 1/4-strength and 1/8-strength MS media without sucrose were most effective for PLB preservation, maintaining viability while minimizing medium darkening and basal browning throughout the 12-month period. For PLB proliferation, 30&#xa0;mg/L ADS facilitated the highest shoot regeneration and PLB production during the first month. After two months, the optimal concentrations of AgNO₃ (1–2&#xa0;mg/L) significantly enhanced PLB multiplication, shoot formation, and root development, while reducing browning and mortality. These results highlight the potential of <i>Spathiphyllum</i> PLBs as a sustainable platform for tissue culture propagation and mid-term conservation. This method is particularly valuable for maintaining stock cultures in large-scale propagation, with promising applications in ornamental horticulture.</p>

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Optimizing protocorm-like body preservation and proliferation in Spathiphyllum wallisii for sustainable micropropagation

  • 期刊论文

  • 发表日期：

    2025年6月30日

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  • Imane Boudadi,
  • Khadija Lachguer,
  • Soumaya El Merzougui,
  • Khalid Lagram,
  • Rachida El boullani,
  • Mohammed Amine Serghini

摘要

Spathiphyllum wallisii, a widely admired ornamental plant, is renowned for its beauty and air-purifying abilities, making it a prominent candidate for tissue culture propagation. Although significant progress has been made in Spathiphyllum micropropagation, the potential of protocorm-like bodies (PLBs) for both propagation and mid-term conservation remains underexplored. In this study, we investigated the effects of varying concentrations of MS medium and sucrose on PLB preservation using slow-growth storage over a 12-month period. Additionally, we optimized PLB proliferation by testing different concentrations of silver nitrate (AgNO₃) and adenine sulphate (ADS). Our findings revealed that 1/4-strength and 1/8-strength MS media without sucrose were most effective for PLB preservation, maintaining viability while minimizing medium darkening and basal browning throughout the 12-month period. For PLB proliferation, 30 mg/L ADS facilitated the highest shoot regeneration and PLB production during the first month. After two months, the optimal concentrations of AgNO₃ (1–2 mg/L) significantly enhanced PLB multiplication, shoot formation, and root development, while reducing browning and mortality. These results highlight the potential of Spathiphyllum PLBs as a sustainable platform for tissue culture propagation and mid-term conservation. This method is particularly valuable for maintaining stock cultures in large-scale propagation, with promising applications in ornamental horticulture.