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<p><i>Curcuma aeruginosa</i> Roxb. is a medicinal herb with significant therapeutic potential. This research aimed to optimize a strategy for micropropagating <i>C. aeruginosa</i> utilizing rhizome buds as an explant and to evaluate the genetic homogeneity of regenerated plants. Healthy sprouting rhizome buds were cultured on Murashige-Skoog (MS) media treated with different concentrations of auxins and cytokinins for shoot and root development, then hardened in various combinations of substrates. The genetic fidelity of micropropagated plantlets and mother plants was assessed using ten primers for each RAPD and ISSR marker. The highest shoot multiplication was found in MS medium enriched with 2.5&#xa0;mg/L BAP and 0.5&#xa0;mg/L NAA, resulting in 11.10 shoots per explant. MS media containing 0.5&#xa0;mg/L IBA was found to be superior for new root development (5.5 roots/explant). Seven of the ten substrate combinations resulted in 100% plantlet survival during the acclimatization period. Cocopeat alone was the most effective substrate for the maximum overall growth of regenerated plants. The genetic integrity of the micropropagated plants was found to be conserved with the wild plants when analyzed using two different molecular markers: RAPD and ISSR markers. Altogether 13 out of twenty primers amplified a total of 43 loci in a consistent pattern ranging in size from 100–1300 base pairs. This research can ensure mass propagation and commercial use of <i>C. aeruginosa</i> for sustainable conservation. Furthermore, it could be crucial to commercial agriculture and facilitating <i>C. aeruginosa</i> certification and regulation compliance.</p>

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Micropropagation and genetic homogeneity assessment of Curcuma aeruginosa Roxb.

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Puskar Basyal,
  • Sabari Rajbahak,
  • Sibesh Maharjhan,
  • Manisha Ghimire,
  • Chandra Bahadur Thapa,
  • Bijaya Pant

摘要

Curcuma aeruginosa Roxb. is a medicinal herb with significant therapeutic potential. This research aimed to optimize a strategy for micropropagating C. aeruginosa utilizing rhizome buds as an explant and to evaluate the genetic homogeneity of regenerated plants. Healthy sprouting rhizome buds were cultured on Murashige-Skoog (MS) media treated with different concentrations of auxins and cytokinins for shoot and root development, then hardened in various combinations of substrates. The genetic fidelity of micropropagated plantlets and mother plants was assessed using ten primers for each RAPD and ISSR marker. The highest shoot multiplication was found in MS medium enriched with 2.5 mg/L BAP and 0.5 mg/L NAA, resulting in 11.10 shoots per explant. MS media containing 0.5 mg/L IBA was found to be superior for new root development (5.5 roots/explant). Seven of the ten substrate combinations resulted in 100% plantlet survival during the acclimatization period. Cocopeat alone was the most effective substrate for the maximum overall growth of regenerated plants. The genetic integrity of the micropropagated plants was found to be conserved with the wild plants when analyzed using two different molecular markers: RAPD and ISSR markers. Altogether 13 out of twenty primers amplified a total of 43 loci in a consistent pattern ranging in size from 100–1300 base pairs. This research can ensure mass propagation and commercial use of C. aeruginosa for sustainable conservation. Furthermore, it could be crucial to commercial agriculture and facilitating C. aeruginosa certification and regulation compliance.