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<p>Anthocyanins, as water-soluble flavonoid pigments, play a crucial role in the growth and metabolic processes of plants, determining the petal color of most angiosperms. In <i>Primulina</i>, belonging to the Gesneriaceae family, anthocyanins are primarily accumulated in the petals, making them unique blue-purple. In previous studies, <i>AN11-like</i> gene, encoding a WD40 protein transcription factor, together with MYB and bHLH transcription factors, controls the synthesis of anthocyanins in some plants. In <i>P. swinglei</i>, the R2R3-MYB transcription factor PswMYB1 is involved in the synthesis of anthocyanins by directly regulating the expression of the <i>PsANS</i>. However, whether <i>AN11-like</i> gene is involved in anthocyanin synthesis in the genus of <i>Primulina</i> has not yet been reported. In this study, we isolated the full-length <i>PscAN11</i> cDNA in <i>P. sclerophylla</i>. PscAN11 protein is localized to the nucleus. <i>PscAN11</i> exhibited high transcriptional level in floral organs, particularly during the pigmentation stage of flower development. Overexpression of <i>PscAN11</i> in tobacco resulted in altered floral color and a significant increase in anthocyanin content, indicating that <i>PscAN11</i> could play a role in the anthocyanin biosynthesis of <i>P. sclerophylla</i>. By employing yeast two-hybrid and Bimolecular Fluorescence Complementation (BiFC) assays, we confirmed the interaction between PscAN11 and PscMYB1, which is a homologous protein of PswMYB1. These results indicated that <i>PscAN11</i> positively regulates the anthocyanin biosynthesis in <i>P. sclerophylla</i> flowers by interacting with PscMYB1.</p>

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PscAN11-encoded WD40 transcription factor controls anthocyanin biosynthesis in the petals of Primulina sclerophylla

  • 期刊论文

  • 发表日期：

    2025年6月3日

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  • Jiajun Huang,
  • Guojian Liang,
  • Jiahao Cao,
  • Chenxi Wang,
  • Lu Liu,
  • Jieni Li,
  • Juanxu Liu,
  • Yixun Yu

摘要

Anthocyanins, as water-soluble flavonoid pigments, play a crucial role in the growth and metabolic processes of plants, determining the petal color of most angiosperms. In Primulina, belonging to the Gesneriaceae family, anthocyanins are primarily accumulated in the petals, making them unique blue-purple. In previous studies, AN11-like gene, encoding a WD40 protein transcription factor, together with MYB and bHLH transcription factors, controls the synthesis of anthocyanins in some plants. In P. swinglei, the R2R3-MYB transcription factor PswMYB1 is involved in the synthesis of anthocyanins by directly regulating the expression of the PsANS. However, whether AN11-like gene is involved in anthocyanin synthesis in the genus of Primulina has not yet been reported. In this study, we isolated the full-length PscAN11 cDNA in P. sclerophylla. PscAN11 protein is localized to the nucleus. PscAN11 exhibited high transcriptional level in floral organs, particularly during the pigmentation stage of flower development. Overexpression of PscAN11 in tobacco resulted in altered floral color and a significant increase in anthocyanin content, indicating that PscAN11 could play a role in the anthocyanin biosynthesis of P. sclerophylla. By employing yeast two-hybrid and Bimolecular Fluorescence Complementation (BiFC) assays, we confirmed the interaction between PscAN11 and PscMYB1, which is a homologous protein of PswMYB1. These results indicated that PscAN11 positively regulates the anthocyanin biosynthesis in P. sclerophylla flowers by interacting with PscMYB1.