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<p>This study investigated the effect of 10 µM copper sulfate (CuSO₄) on the micropropagation of <i>Miscanthus × giganteus</i>. The results showed that CuSO₄ supplementation enhances regeneration efficiency and phenolic content in long-term callus cultures of <i>M. × giganteus</i>, with notable cultivar-specific differences. The ‘Nagara’ cultivar exhibited a higher regeneration rate, increasing from 26 to 43% after six months of CuSO₄ supplementation, and maintained this potential after 12 months. In contrast, the ‘Illinois’ cultivar showed regeneration capability only during the six-month culture period, with no regeneration observed under control conditions. Phenolic content increased significantly during CuSO₄ supplementation, particularly in the ‘Illinois’ cultivar, where a fivefold increase was observed after 12 months. Monitoring the influence of CuSO₄ over time showed that 18-month-old plants from both cultivars, derived from the CuSO₄-supplemented cultures, exhibited improved physiological traits, with plant biometric parameters remaining intact. The observed trend supports the possibility of incorporating the in vitro propagation of <i>Miscanthus × giganteus</i> on a larger scale while maintaining optimal growth. These results also rule out any negative effects of the proposed protocol on the biomass production efficiency making it a promising solution for <i>Miscanthus</i> cultivation. Our study highlights the role of CuSO₄ in sustaining regeneration and provides a valuable tool for optimizing propagation protocols for bioenergy crops.</p>

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Enhanced micropropagation of Miscanthus × giganteus: a sustainable approach to optimal regenerated plant growth

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月31日

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  • Estera Wojtkowiak,
  • Katarzyna B. Czyż,
  • Aneta Basińska-Barczak,
  • Hanna Pudelska,
  • Katarzyna Głowacka,
  • Tomasz Pniewski,
  • Karolina Sobańska

摘要

This study investigated the effect of 10 µM copper sulfate (CuSO₄) on the micropropagation of Miscanthus × giganteus. The results showed that CuSO₄ supplementation enhances regeneration efficiency and phenolic content in long-term callus cultures of M. × giganteus, with notable cultivar-specific differences. The ‘Nagara’ cultivar exhibited a higher regeneration rate, increasing from 26 to 43% after six months of CuSO₄ supplementation, and maintained this potential after 12 months. In contrast, the ‘Illinois’ cultivar showed regeneration capability only during the six-month culture period, with no regeneration observed under control conditions. Phenolic content increased significantly during CuSO₄ supplementation, particularly in the ‘Illinois’ cultivar, where a fivefold increase was observed after 12 months. Monitoring the influence of CuSO₄ over time showed that 18-month-old plants from both cultivars, derived from the CuSO₄-supplemented cultures, exhibited improved physiological traits, with plant biometric parameters remaining intact. The observed trend supports the possibility of incorporating the in vitro propagation of Miscanthus × giganteus on a larger scale while maintaining optimal growth. These results also rule out any negative effects of the proposed protocol on the biomass production efficiency making it a promising solution for Miscanthus cultivation. Our study highlights the role of CuSO₄ in sustaining regeneration and provides a valuable tool for optimizing propagation protocols for bioenergy crops.