Scholarlink

<p><i>Cymbidium bicolor</i> Lindl., a rare epiphytic orchid valued for its ethnomedicinal properties. This study aimed to develop an efficient in vitro propagation protocol and evaluate certain pharmaceutical potential metabolites of the regenerated plants. Species identity was confirmed through morphotaxonomic characters as well as molecular characterization using <i>matK</i> and ITS markers. Optimal asymbiotic seed germination (95.48%) was achieved from 11-month-after-pollination immature green pods cultured on Murashige and Skoog (MS) medium supplemented with 2% sucrose and 9&#xa0;µM/L IAA. Subsequent protocorm differentiation into rooted plantlets was maximized (7.12 plantlets per protocorm) on MS medium fortified with 3% sucrose and a combination of 6&#xa0;µM/L BAP and 3&#xa0;µM/L NAA. Genetic fidelity assessment using RAPD, DAMD, and SCoT markers revealed high genetic uniformity among the regenerates, exhibiting 96.57% similarity to the donor plant based on Jaccard's coefficient analysis. Quantification of key phytochemicals demonstrated significantly higher concentrations of total polyphenol content (8.67&#xa0;mg GAE/g), total flavonoid content (27.73&#xa0;mg QE/g), total tannins (11.95&#xa0;mg TAE/g), and total alkaloids (4.83&#xa0;mg AE/g) in the in vitro regenerated plants compared to leaf, pseudobulb, and root extracts of the mother plant along with superior radical scavenging activity in DPPH, FRAP, and ABTS assays. This study provides an efficient in vitro propagation protocol for the conservation and mass production of genetically uniform plantlets. The enhanced accumulation of valuable phytochemicals and potent antioxidant activity in the regenerated plants highlights the potential of tissue culture as a sustainable strategy for both species preservation and the production of high-value medicinal compounds.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Efficient in vitro protocol for immature seed germination and regeneration of Cymbidium bicolor Lindl. plants with enhanced phytochemicals and antioxidant properties

  • 期刊论文

  • 发表日期：

    2025年6月24日

查看全文
  • Temjennokcha B. Longchar,
  • Chitta Ranjan Deb

摘要

Cymbidium bicolor Lindl., a rare epiphytic orchid valued for its ethnomedicinal properties. This study aimed to develop an efficient in vitro propagation protocol and evaluate certain pharmaceutical potential metabolites of the regenerated plants. Species identity was confirmed through morphotaxonomic characters as well as molecular characterization using matK and ITS markers. Optimal asymbiotic seed germination (95.48%) was achieved from 11-month-after-pollination immature green pods cultured on Murashige and Skoog (MS) medium supplemented with 2% sucrose and 9 µM/L IAA. Subsequent protocorm differentiation into rooted plantlets was maximized (7.12 plantlets per protocorm) on MS medium fortified with 3% sucrose and a combination of 6 µM/L BAP and 3 µM/L NAA. Genetic fidelity assessment using RAPD, DAMD, and SCoT markers revealed high genetic uniformity among the regenerates, exhibiting 96.57% similarity to the donor plant based on Jaccard's coefficient analysis. Quantification of key phytochemicals demonstrated significantly higher concentrations of total polyphenol content (8.67 mg GAE/g), total flavonoid content (27.73 mg QE/g), total tannins (11.95 mg TAE/g), and total alkaloids (4.83 mg AE/g) in the in vitro regenerated plants compared to leaf, pseudobulb, and root extracts of the mother plant along with superior radical scavenging activity in DPPH, FRAP, and ABTS assays. This study provides an efficient in vitro propagation protocol for the conservation and mass production of genetically uniform plantlets. The enhanced accumulation of valuable phytochemicals and potent antioxidant activity in the regenerated plants highlights the potential of tissue culture as a sustainable strategy for both species preservation and the production of high-value medicinal compounds.