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<p><i>Kaempferia parviflora</i> is a valued medicinal plant in Indian traditional medicine known for its healing qualities, but its native population faces stress due to limited planting material and poor regeneration. Building on this principle, the study investigates key factors affecting micro-rhizome induction, the primary regenerative component. With a constant sucrose concentration of 60&#xa0;g/L, we examined 29 distinct concentrations of plant growth regulators to determine the ideal conditions. Compared to 30 and 60 days of inoculation, the micro-rhizomes reached their maximum size (1.13&#xa0;cm), developed a woody texture, and exhibited the highest abundance (17.5%) after 90 days. The optimal combination was observed in Murashige and Skoog (MS) medium supplemented with 60&#xa0;g/L sucrose, 1&#xa0;mg/L each of IAA, and BAP, which yielded the highest number of in vitro micro-rhizomes. Consequently, this medium composition was standardized. In vitro variations in micro-rhizome development were analysed based on culture containers, sucrose concentrations, and MS medium types (solid and liquid), all supplemented with the same hormone combination (1&#xa0;mg/L IAA + 1&#xa0;mg/L BAP). The propagation rate was higher in semisolid medium (66%) compared to liquid medium (34%), reinforcing the suitability of semisolid conditions. Among the culture containers tested, test tubes demonstrated the most rapid and consistent micro-rhizome growth (9.73%). Sucrose concentration also played a crucial role, with 60&#xa0;g/L yielding the highest micro-rhizome quantity (8.53%) and size (0.85&#xa0;cm), while 30&#xa0;g/L sucrose promoted optimal shoot and root length. The in vitro-grown plantlets were acclimatized in a shade house before being transplanted to the field, achieving an impressive 100% survival rate. Furthermore, ISSR analysis confirmed genetic stability, as no variations were detected between the mother plants and micropropagated plants. Micro-rhizome induction offers a sustainable alternative to reduce overexploitation while meeting industrial and public demands.</p>

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An improved protocol for in vitro induction of micro-rhizome in Kaempferia parviflora Wall. ex Baker (Zingiberaceae) and assessment of genetic stability using ISSR markers

  • 期刊论文

  • 发表日期：

    2025年8月26日

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  • Disianliu Gangmei,
  • Thokchom Amumacha Singh,
  • Rakesh Sanabam,
  • Nandeibam Samarjit Singh,
  • Huidrom Sunitibala Devi

摘要

Kaempferia parviflora is a valued medicinal plant in Indian traditional medicine known for its healing qualities, but its native population faces stress due to limited planting material and poor regeneration. Building on this principle, the study investigates key factors affecting micro-rhizome induction, the primary regenerative component. With a constant sucrose concentration of 60 g/L, we examined 29 distinct concentrations of plant growth regulators to determine the ideal conditions. Compared to 30 and 60 days of inoculation, the micro-rhizomes reached their maximum size (1.13 cm), developed a woody texture, and exhibited the highest abundance (17.5%) after 90 days. The optimal combination was observed in Murashige and Skoog (MS) medium supplemented with 60 g/L sucrose, 1 mg/L each of IAA, and BAP, which yielded the highest number of in vitro micro-rhizomes. Consequently, this medium composition was standardized. In vitro variations in micro-rhizome development were analysed based on culture containers, sucrose concentrations, and MS medium types (solid and liquid), all supplemented with the same hormone combination (1 mg/L IAA + 1 mg/L BAP). The propagation rate was higher in semisolid medium (66%) compared to liquid medium (34%), reinforcing the suitability of semisolid conditions. Among the culture containers tested, test tubes demonstrated the most rapid and consistent micro-rhizome growth (9.73%). Sucrose concentration also played a crucial role, with 60 g/L yielding the highest micro-rhizome quantity (8.53%) and size (0.85 cm), while 30 g/L sucrose promoted optimal shoot and root length. The in vitro-grown plantlets were acclimatized in a shade house before being transplanted to the field, achieving an impressive 100% survival rate. Furthermore, ISSR analysis confirmed genetic stability, as no variations were detected between the mother plants and micropropagated plants. Micro-rhizome induction offers a sustainable alternative to reduce overexploitation while meeting industrial and public demands.