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<p>Hybrids frequently outperform traditional varieties due to their enhanced yield and robust stress tolerance. A critical step in hybrid seed production is the development of homozygous inbred lines. To expedite the breeding process, we employed the isolated microspore culture (IMC) technique to obtain doubled haploid (DH) lines, but the rate of some microspore embryogenesis was extremely low. This study was aimed at establishing an effective microspore culture program for recalcitrant genotypes of pakchoi. We selected N-(2-Chloro-4-pyridyl)-N’-phenyl urea (CPPU) as an inducer for microspore embryogenesis since it was reported that CPPU could promote plant cell division. Three pakchoi lines 21Q04, 21Q08 and 21Q10 were used to test the CPPU effects. Results indicated that the most efficient CPPU concentration to enhance microspore embryogenesis was 0.01&#xa0;mg/L; and at that concentration, the embryo yields of the three genotypes were 5.54, 3.22 and 2.43 times higher than those of control, respectively. The frequency of direct seedling formation was promoted by changing the inoculation direction of embryos in MS solid medium. These measures facilitate the application of microspore cultivation in pakchoi breeding.</p>

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N-(2-Chloro-4-pyridyl)-N’-phenylurea promotes microspore embryogenesis and plant regeneration in pakchoi (Brassica rapa L. ssp. chinensis)

  • 期刊论文

  • 发表日期：

    2025年5月13日

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  • Xue Li,
  • Yuanzhi Bai,
  • Zhiyin Huang,
  • Weiqiang Fan,
  • Nan Wang,
  • Hui Feng

摘要

Hybrids frequently outperform traditional varieties due to their enhanced yield and robust stress tolerance. A critical step in hybrid seed production is the development of homozygous inbred lines. To expedite the breeding process, we employed the isolated microspore culture (IMC) technique to obtain doubled haploid (DH) lines, but the rate of some microspore embryogenesis was extremely low. This study was aimed at establishing an effective microspore culture program for recalcitrant genotypes of pakchoi. We selected N-(2-Chloro-4-pyridyl)-N’-phenyl urea (CPPU) as an inducer for microspore embryogenesis since it was reported that CPPU could promote plant cell division. Three pakchoi lines 21Q04, 21Q08 and 21Q10 were used to test the CPPU effects. Results indicated that the most efficient CPPU concentration to enhance microspore embryogenesis was 0.01 mg/L; and at that concentration, the embryo yields of the three genotypes were 5.54, 3.22 and 2.43 times higher than those of control, respectively. The frequency of direct seedling formation was promoted by changing the inoculation direction of embryos in MS solid medium. These measures facilitate the application of microspore cultivation in pakchoi breeding.