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<p>Shaker K⁺ channels play a vital role in plant potassium homeostasis and stress adaptation. While existing research predominantly focuses on model plants like Arabidopsis and agricultural crops, investigations into Shaker K⁺ channels in grass species remain limited. Given the potential for distinct characteristics in grass Shaker K⁺ channel gene families, this study conducted a comprehensive genome-wide analysis of these channels in centipedegrass, a species widely used in landscaping. Seven Shaker K⁺ channel genes were identified, unevenly distributed across five chromosomes. Notably, centipedegrass harbors fewer members compared to other species, due to the absence of group III and IV subfamilies. Structural variations among these genes suggest functional diversity. Functional assays in potassium-deficient yeast revealed that only EoKAT1 and EoKAT2 displayed potassium uptake capabilities, with EoKAT1 exhibiting markedly higher absorption than EoKAT2. Surprisingly, EoAKT1 failed to demonstrate potassium absorption functionality, contrasting with its homologs in other species. Salt tolerance assays in yeast further showed that EoKAT1 significantly enhanced cellular salt tolerance, while EoKAT2 had no effect—a finding consistent with their differential potassium uptake capacities. EoKAT1 and EoKAT2 share a collinear relationship, and disparities in their potassium absorption, salt tolerance, and promoter elements highlight functional divergence, potentially enabling distinct environmental responses. Subcellular localization confirmed EoKAT1 is anchored to the plasma membrane. Collectively, this study provides insights into the unique characteristics of the Shaker K⁺ channel family in centipedegrass, differing from other species. EoKAT1 emerges as a promising candidate for improving plant potassium utilization and salt stress resilience.</p>

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Genome-wide identification and functional characterization of the Shaker potassium channel family in centipedegrass suggest their distinct roles in potassium uptake and salt tolerance

  • 期刊论文

  • 发表日期：

    2025年5月14日

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  • Dong-Li Hao,
  • Jin-Yan Zhou,
  • Jia Qu,
  • Jun-Yi Zhai,
  • Ying Zhao,
  • Jian-Xiu Liu,
  • Hai-Lin Guo,
  • Jun-Qin Zong

摘要

Shaker K⁺ channels play a vital role in plant potassium homeostasis and stress adaptation. While existing research predominantly focuses on model plants like Arabidopsis and agricultural crops, investigations into Shaker K⁺ channels in grass species remain limited. Given the potential for distinct characteristics in grass Shaker K⁺ channel gene families, this study conducted a comprehensive genome-wide analysis of these channels in centipedegrass, a species widely used in landscaping. Seven Shaker K⁺ channel genes were identified, unevenly distributed across five chromosomes. Notably, centipedegrass harbors fewer members compared to other species, due to the absence of group III and IV subfamilies. Structural variations among these genes suggest functional diversity. Functional assays in potassium-deficient yeast revealed that only EoKAT1 and EoKAT2 displayed potassium uptake capabilities, with EoKAT1 exhibiting markedly higher absorption than EoKAT2. Surprisingly, EoAKT1 failed to demonstrate potassium absorption functionality, contrasting with its homologs in other species. Salt tolerance assays in yeast further showed that EoKAT1 significantly enhanced cellular salt tolerance, while EoKAT2 had no effect—a finding consistent with their differential potassium uptake capacities. EoKAT1 and EoKAT2 share a collinear relationship, and disparities in their potassium absorption, salt tolerance, and promoter elements highlight functional divergence, potentially enabling distinct environmental responses. Subcellular localization confirmed EoKAT1 is anchored to the plasma membrane. Collectively, this study provides insights into the unique characteristics of the Shaker K⁺ channel family in centipedegrass, differing from other species. EoKAT1 emerges as a promising candidate for improving plant potassium utilization and salt stress resilience.