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<p>Increasing evidence suggests interconnections between hydroxycinnamate-derived compounds and the developmental stages of a plant cell during vital processes such as response to stress factors, auxin transportation, cell wall reinforcement, and differentiation. The fine-tuning of these phenomena, which can vary significantly between different plant cell cultures, makes nondestructive monitoring an essential practice in contemporary biotechnology. Fluorescence emissions of chlorophyll, phenolic compounds, and other fluorophores of plant leaves have been profoundly investigated. Now, there is a tendency toward extending the application of this technology to plant biotechnology where the plant cells and hairy roots usually lack chlorophyll. Considering the autofluorescence of phenolic compounds, particularly phenolic acids, the fluorescence emission of two <i>Nonea caspica</i> callus (3 and 18 months old) were studied at different time intervals of the 30-day subcultures on Linsmaier and Skoog (LS) medium in darkness. Revisiting the fluorescence emissions spectra of some key compounds of the phenylpropanoid pathway using excitation beams of 330, 375, and 450&#xa0;nm, results of HPLC analysis, and LC-Mass analysis of the hydroalcoholic extracts of the callus helped to decipher the fluorescence spectra of the callus obtained with the nondestructive method set up in this study. The spectra revealed a gradual accumulation of phenolic acids during subculture, dominated by hydroxycinnamic acid derivatives, and incorporation of both feruloyl and sinapoyl units into the cell wall with age-dependent shifting preferences between these components. This approach provides a foundation for real-time studies on phenolic acids in plant cells for both ecophysiological and biotechnological purposes.</p>

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Nondestructive fluorescence spectroscopy for tracking phenolic acids production in cell culture of Nonea caspica

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Kamahldin Haghbeen,
  • Hossein Sheikhi,
  • Mohammad Javad Marefatjoo,
  • Mehdi Nikzad Semeskandi,
  • Jaleh Ghashghaie,
  • Gwendal Latouche,
  • Zoran G. Cerovic

摘要

Increasing evidence suggests interconnections between hydroxycinnamate-derived compounds and the developmental stages of a plant cell during vital processes such as response to stress factors, auxin transportation, cell wall reinforcement, and differentiation. The fine-tuning of these phenomena, which can vary significantly between different plant cell cultures, makes nondestructive monitoring an essential practice in contemporary biotechnology. Fluorescence emissions of chlorophyll, phenolic compounds, and other fluorophores of plant leaves have been profoundly investigated. Now, there is a tendency toward extending the application of this technology to plant biotechnology where the plant cells and hairy roots usually lack chlorophyll. Considering the autofluorescence of phenolic compounds, particularly phenolic acids, the fluorescence emission of two Nonea caspica callus (3 and 18 months old) were studied at different time intervals of the 30-day subcultures on Linsmaier and Skoog (LS) medium in darkness. Revisiting the fluorescence emissions spectra of some key compounds of the phenylpropanoid pathway using excitation beams of 330, 375, and 450 nm, results of HPLC analysis, and LC-Mass analysis of the hydroalcoholic extracts of the callus helped to decipher the fluorescence spectra of the callus obtained with the nondestructive method set up in this study. The spectra revealed a gradual accumulation of phenolic acids during subculture, dominated by hydroxycinnamic acid derivatives, and incorporation of both feruloyl and sinapoyl units into the cell wall with age-dependent shifting preferences between these components. This approach provides a foundation for real-time studies on phenolic acids in plant cells for both ecophysiological and biotechnological purposes.