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<p>Melatonin is a multifunctional molecule active in various metabolic processes, influencing the accumulation of bioactive metabolites in medicinal plants such as <i>Pfaffia glomerata</i>. This species has pharmacological and medicinal properties, mainly due to 20-hydroxyecdysone (20-E) production. This study aimed to evaluate the effect of exogenous melatonin on growth and development, physiology, and 20-E content in <i>P. glomerata in vitro</i> culture. To achieve this, <i>P. glomerata</i> was grown for 35 days in a in vitro culture medium supplemented with 0, 10, 100, 500, and 1000 µM melatonin. Morphophysiological parameters, antioxidant defenses, and 20-E production were subsequently assessed. Melatonin reduced pigment content by 25%, with higher concentrations (≥ 500 µM) negatively affecting plant growth. This was evident in reduced stem length (39% lower), root length (50% shorter than the control), and biomass accumulation in the leaf, stem, and root dry weight, where values reached only 50% of those observed in the control group. However, melatonin also activated antioxidant response mechanisms, resulting in a 100% increase in antioxidant enzyme activity in the leaves. At the same time, it reduced lipid peroxidation in the leaves and H<sub>2</sub>O<sub>2</sub> accumulation in the roots, with a 50% decrease in groups treated with ≥ 500 µM of melatonin. Furthermore, melatonin increased the levels of amino acids (by over 33%), proline (25%), and proteins (100%) in the roots. It also stimulated the production of 20-E (28% higher at 10 µM) and boosted the contents of zeatin (850%), ACC (198%), and methyl jasmonate (150%) in the leaves, all of which are key stress-related hormones. Our findings provide further insight into the role of melatonin as a regulatory molecule in plants, with its effects being concentration-dependent.</p>

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The effect of different concentration of melatonin on physiological and metabolic atributes in Pfaffia glomerata in vitro cultures

  • 期刊论文

  • 发表日期：

    2025年5月1日

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  • José Victor S. Silva,
  • Tatiane D. Silva,
  • Sérgio Heitor S. Felipe,
  • Diego S. Batista,
  • Juliane M. Henschel,
  • Evandro A. Fortini,
  • Letícia M. Farias,
  • João Paulo V. Leite,
  • Michelle Maylla V. Almeida,
  • Kleiton L. G. Machado,
  • Wagner C. Otoni

摘要

Melatonin is a multifunctional molecule active in various metabolic processes, influencing the accumulation of bioactive metabolites in medicinal plants such as Pfaffia glomerata. This species has pharmacological and medicinal properties, mainly due to 20-hydroxyecdysone (20-E) production. This study aimed to evaluate the effect of exogenous melatonin on growth and development, physiology, and 20-E content in P. glomerata in vitro culture. To achieve this, P. glomerata was grown for 35 days in a in vitro culture medium supplemented with 0, 10, 100, 500, and 1000 µM melatonin. Morphophysiological parameters, antioxidant defenses, and 20-E production were subsequently assessed. Melatonin reduced pigment content by 25%, with higher concentrations (≥ 500 µM) negatively affecting plant growth. This was evident in reduced stem length (39% lower), root length (50% shorter than the control), and biomass accumulation in the leaf, stem, and root dry weight, where values reached only 50% of those observed in the control group. However, melatonin also activated antioxidant response mechanisms, resulting in a 100% increase in antioxidant enzyme activity in the leaves. At the same time, it reduced lipid peroxidation in the leaves and H2O2 accumulation in the roots, with a 50% decrease in groups treated with ≥ 500 µM of melatonin. Furthermore, melatonin increased the levels of amino acids (by over 33%), proline (25%), and proteins (100%) in the roots. It also stimulated the production of 20-E (28% higher at 10 µM) and boosted the contents of zeatin (850%), ACC (198%), and methyl jasmonate (150%) in the leaves, all of which are key stress-related hormones. Our findings provide further insight into the role of melatonin as a regulatory molecule in plants, with its effects being concentration-dependent.