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<p>Successful scale-up of <i>Bacopa monnieri</i> cell suspension (CS) culture for production of Bacopa saponins was performed in a stirred tank bioreactor using batch and fed-batch strategies. Mathematical model was developed using bioreactor batch kinetic study data and the model parameters were estimated ((maximum specific growth rate (μ<sub>m</sub> = 0.171 d<sup>−1</sup>), biomass yield per unit substrate consumed (Y<sub>x/s</sub>&#xa0;= 0.34 g/g), maintenance coefficient with respect to substrate consumed (m<sub>s</sub>= 0.235 g/g/day), saturation constant with respect to substrate (K<sub>s</sub>= 15.33 g/l), growth associated product formation constant (K<sub>l</sub> = 14.824 mg/g) and non-growth associated product formation constant (K<sub>2</sub> = 0.904 mg/g/day)) to study the role of substrate limitation at low concentration and substrate inhibition at a very high concentration on production of Bacopa saponins and overall cell metabolism. Fed-batch culture of <i>Bacopa</i> saponins was performed in the bioreactor. The maximum biomass concentration in bioreactor fed-batch culture was 6.57 g/l DW and the corresponding total bacoside A concentration was 89.02 mg/l, respectively. The concentration of individual <i>Bacopa</i> saponins was also considerably higher in fed batch cultivation (27.51 mg/l bacoside A3 and 20.74 mg/l bacopaside II, 9.56 mg/l bacopaside X and 32.51 mg/l bacopasaponin C) as compared to batch culture (21.06 mg/l of bacoside A3, 11.72 mg/l of bacopaside II, 6.39 mg/l bacopaside X and 8.22 mg/l bacopasaponin C). The bioreactor fed-batch culture results were also better than the shake flask batch results under optimized conditions (4.15 g/l biomass on DW basis, 37.32 mg/l total bacoside A). This study can help in commercial production of Bacoside A.</p>

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Production of Bacopa saponins using fed batch studies in bioreactor

  • 期刊论文

  • 发表日期：

    2025年6月11日

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  • Jayashree Nanda,
  • Binod B. Sahu,
  • V. R. Singh,
  • Nivedita Patra

摘要

Successful scale-up of Bacopa monnieri cell suspension (CS) culture for production of Bacopa saponins was performed in a stirred tank bioreactor using batch and fed-batch strategies. Mathematical model was developed using bioreactor batch kinetic study data and the model parameters were estimated ((maximum specific growth rate (μm = 0.171 d−1), biomass yield per unit substrate consumed (Yx/s = 0.34 g/g), maintenance coefficient with respect to substrate consumed (ms= 0.235 g/g/day), saturation constant with respect to substrate (Ks= 15.33 g/l), growth associated product formation constant (Kl = 14.824 mg/g) and non-growth associated product formation constant (K2 = 0.904 mg/g/day)) to study the role of substrate limitation at low concentration and substrate inhibition at a very high concentration on production of Bacopa saponins and overall cell metabolism. Fed-batch culture of Bacopa saponins was performed in the bioreactor. The maximum biomass concentration in bioreactor fed-batch culture was 6.57 g/l DW and the corresponding total bacoside A concentration was 89.02 mg/l, respectively. The concentration of individual Bacopa saponins was also considerably higher in fed batch cultivation (27.51 mg/l bacoside A3 and 20.74 mg/l bacopaside II, 9.56 mg/l bacopaside X and 32.51 mg/l bacopasaponin C) as compared to batch culture (21.06 mg/l of bacoside A3, 11.72 mg/l of bacopaside II, 6.39 mg/l bacopaside X and 8.22 mg/l bacopasaponin C). The bioreactor fed-batch culture results were also better than the shake flask batch results under optimized conditions (4.15 g/l biomass on DW basis, 37.32 mg/l total bacoside A). This study can help in commercial production of Bacoside A.