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<p>Ethylene Response Factor (ERF) transcription factors are pivotal in enhancing plant resistance to adverse environmental conditions. Previous research has indicated that LkERF6 significantly improves drought resistance in transgenic tobacco; however, the specific downstream genes regulated by LkERF6 remain poorly understood. In the present research, we analyzed the transcriptome from leaf tissues of wild-type (WT) and <i>LkERF6-</i>overexpressing transgenic tobacco exposed to drought stress. Compared to WT, the LkERF6 group exhibited 2113 differentially expressed genes (DEGs), including 841 significantly downregulated and 1292 upregulated genes. Gene Ontology (GO) analysis revealed that LkERF6 is primarily associated with biological processes including photosynthesis, defense response, cell wall biosynthesis, signal transduction, sugar metabolism, fatty acid metabolism, and amino acid metabolism. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that LkERF6 is predominantly associated with signaling pathways related to environmental information processing, carbohydrate metabolism, energy metabolism, lipid metabolism, and amino acid metabolism. Furthermore, within the reactive oxygen species (ROS) clearance, cell wall biosynthesis, signal transduction, and abscisic acid (ABA) pathways, numerous genes were markedly upregulated in the LkERF6 group, including peroxidase (PRX), xyloglucosyltransferase/hydrolase (XTH), cellulose synthase (CES), ABC transporter (ABCC/ABCG), and ABA receptor (PYL). These findings suggest that LkERF6 may activate the transcription of these genes to enhance drought resistance. This study elucidates the downstream genes regulated by LkERF6 and provides insights into the molecular mechanisms underlying LkERF6’s function in <i>Larix kaempferi</i>.&#xa0;</p> Graphical Abstract <p></p>

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Transcriptomic analysis reveals the drought tolerance mechanism of Larix kaempferi ERF6 (LkERF6) in Nicotiana benthamiana

  • 期刊论文

  • 发表日期：

    2025年6月20日

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  • Ming Tian,
  • Junying Lu,
  • Yuqian Wang,
  • Xiangning Jiang,
  • Ying Gai

摘要

Ethylene Response Factor (ERF) transcription factors are pivotal in enhancing plant resistance to adverse environmental conditions. Previous research has indicated that LkERF6 significantly improves drought resistance in transgenic tobacco; however, the specific downstream genes regulated by LkERF6 remain poorly understood. In the present research, we analyzed the transcriptome from leaf tissues of wild-type (WT) and LkERF6-overexpressing transgenic tobacco exposed to drought stress. Compared to WT, the LkERF6 group exhibited 2113 differentially expressed genes (DEGs), including 841 significantly downregulated and 1292 upregulated genes. Gene Ontology (GO) analysis revealed that LkERF6 is primarily associated with biological processes including photosynthesis, defense response, cell wall biosynthesis, signal transduction, sugar metabolism, fatty acid metabolism, and amino acid metabolism. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that LkERF6 is predominantly associated with signaling pathways related to environmental information processing, carbohydrate metabolism, energy metabolism, lipid metabolism, and amino acid metabolism. Furthermore, within the reactive oxygen species (ROS) clearance, cell wall biosynthesis, signal transduction, and abscisic acid (ABA) pathways, numerous genes were markedly upregulated in the LkERF6 group, including peroxidase (PRX), xyloglucosyltransferase/hydrolase (XTH), cellulose synthase (CES), ABC transporter (ABCC/ABCG), and ABA receptor (PYL). These findings suggest that LkERF6 may activate the transcription of these genes to enhance drought resistance. This study elucidates the downstream genes regulated by LkERF6 and provides insights into the molecular mechanisms underlying LkERF6’s function in Larix kaempferi. 

Graphical Abstract