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<p>This study investigated the antioxidant potential of callus cultures derived from the endangered medicinal plant <i>Ferula tadshikorum</i> Pimenov (Apiaceae Lindl.), an endemic species in Central Asia. The plant’s long life cycle and overharvesting threaten its survival, necessitating the development of in vitro microclonal propagation protocols as a sustainable solution for bioactive compound production. Two propagation methods, indirect organogenesis and indirect somatic embryogenesis, were optimized using zygotic embryos as explants. Callus cultures at early stages, cultivated on Murashige and Skoog medium supplemented with 0.5&#xa0;mg/l 2,4-dichlorophenoxyacetic acid, demonstrated antioxidant activity, with 2,2-diphenyl-1-picrylhydrazyl (DPPH) half-maximal inhibitory concentration (IC₅₀) values as low as 15.41&#xa0;µg/mL, comparable to ascorbic acid (15.17&#xa0;µg/mL), while the radical-scavenging activity in the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay was even higher. Optimal bioactivity and extract yields were achieved on a Murashige and Skoog medium supplemented with 2.4-dichlorophenoxyacetic acid (0.5&#xa0;mg/l) and Kinetin or Thidiazuron. Statistical analysis confirmed the influence of hormonal treatments on antioxidant properties. These findings highlight the dual potential of in vitro propagation for conserving <i>F. tadshikorum</i> and providing a sustainable source of bioactive compounds with significant antioxidant properties, supporting pharmaceutical applications. However, challenges in translating these findings into large-scale production or commercial applications must be addressed, including the optimization of protocols for industrial scalability and cost-effectiveness.</p>

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Optimized microclonal propagation protocol and antioxidant activity of callus cultures from the endangered Ferula tadshikorum Pimenov (Apiaceae Lindl.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月12日

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  • Feruza Usmanovna Mustafina,
  • Dilafruz Nematilla kizi Jamalova,
  • Amir Reza Zarekarizi,
  • Hanifabonu Kobul kizi Juraeva,
  • Abbos Tulkin ogli Khazratov,
  • Kim Hoe Jin,
  • Na Chae Sun,
  • Lee Min Sung,
  • Oh Yu Jin,
  • Komiljon Sharobiddinovich Tojibaev,
  • Sodikjon Khaliknazarovich Abdinazarov,
  • Jong Tae Lim

摘要

This study investigated the antioxidant potential of callus cultures derived from the endangered medicinal plant Ferula tadshikorum Pimenov (Apiaceae Lindl.), an endemic species in Central Asia. The plant’s long life cycle and overharvesting threaten its survival, necessitating the development of in vitro microclonal propagation protocols as a sustainable solution for bioactive compound production. Two propagation methods, indirect organogenesis and indirect somatic embryogenesis, were optimized using zygotic embryos as explants. Callus cultures at early stages, cultivated on Murashige and Skoog medium supplemented with 0.5 mg/l 2,4-dichlorophenoxyacetic acid, demonstrated antioxidant activity, with 2,2-diphenyl-1-picrylhydrazyl (DPPH) half-maximal inhibitory concentration (IC₅₀) values as low as 15.41 µg/mL, comparable to ascorbic acid (15.17 µg/mL), while the radical-scavenging activity in the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay was even higher. Optimal bioactivity and extract yields were achieved on a Murashige and Skoog medium supplemented with 2.4-dichlorophenoxyacetic acid (0.5 mg/l) and Kinetin or Thidiazuron. Statistical analysis confirmed the influence of hormonal treatments on antioxidant properties. These findings highlight the dual potential of in vitro propagation for conserving F. tadshikorum and providing a sustainable source of bioactive compounds with significant antioxidant properties, supporting pharmaceutical applications. However, challenges in translating these findings into large-scale production or commercial applications must be addressed, including the optimization of protocols for industrial scalability and cost-effectiveness.