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<p><i>Larix kaempferi</i>, one of the representative gymnosperms, is the main afforestation and timber species in the world. As one essential micronutrient, zinc plays a vital role in <i>Larix kaempferi</i> growth, yet its overaccumulation causes substantial growth inhibition. However, the underlying mechanism of zinc stress response in <i>Larix kaempferi</i> is still not well elucidated. In the present study, a potential zinc stress-related gene, <i>LkCNR6</i> (<i>Cell number regulator 6</i>) was identified in <i>Larix kaempferi</i>. <i>LkCNR6</i> was confirmed to localize in the plasma membrane and displayed rapidly inducible expression patterns under zinc stress. To uncover the roles of <i>LkCNR6</i>, the <i>LkCNR6</i>-overexpressing transgenic <i>Arabidopsis</i> lines were created. The phenotypic data confirmed that ectopic overexpression of <i>LkCNR6</i> significantly improved zinc stress tolerance in <i>Arabidopsis</i>, although the <i>LkCNR6</i>-overexpressing transgenic plants displayed slower growth rate and smaller aerial organ size than that of the wild-type plants. Zinc accumulation assay indicated that the improved tolerance to zinc stress was mostly attributed to increase zinc excretion in the overexpressing <i>LkCNR6</i> transgenic plants. Furthermore, yeast two-hybrid assay identified that LkHMP1, LkWAKL4 and LkRTFL, were physically interacted with LkCNR6. The interaction between LkCNR6 and LkHMP1, a protein associated with metal transport, was further confirmed by bimolecular fluorescence complementation (BiFC) assay. In summary, these results demonstrated that <i>LkCNR6</i> plays important roles in positively responding zinc stress and negatively regulating organ development. These findings provide new insights into the regulatory mechanism of <i>LkCNR6</i>-mediated zinc stress response, and suggest the application of <i>LkCNR6</i> in breeding high-heavy metal resistance crops.</p>

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LkCNR6, a member of Cell number regulator genes from Larix kaempferi, confers zinc stress tolerance in transgenic Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • Xu Zhang,
  • Yu Li,
  • Chengrong Yun,
  • Wenting Sun,
  • Zhoupeng Fan,
  • Yunhui Xie,
  • Chunguo Wang

摘要

Larix kaempferi, one of the representative gymnosperms, is the main afforestation and timber species in the world. As one essential micronutrient, zinc plays a vital role in Larix kaempferi growth, yet its overaccumulation causes substantial growth inhibition. However, the underlying mechanism of zinc stress response in Larix kaempferi is still not well elucidated. In the present study, a potential zinc stress-related gene, LkCNR6 (Cell number regulator 6) was identified in Larix kaempferi. LkCNR6 was confirmed to localize in the plasma membrane and displayed rapidly inducible expression patterns under zinc stress. To uncover the roles of LkCNR6, the LkCNR6-overexpressing transgenic Arabidopsis lines were created. The phenotypic data confirmed that ectopic overexpression of LkCNR6 significantly improved zinc stress tolerance in Arabidopsis, although the LkCNR6-overexpressing transgenic plants displayed slower growth rate and smaller aerial organ size than that of the wild-type plants. Zinc accumulation assay indicated that the improved tolerance to zinc stress was mostly attributed to increase zinc excretion in the overexpressing LkCNR6 transgenic plants. Furthermore, yeast two-hybrid assay identified that LkHMP1, LkWAKL4 and LkRTFL, were physically interacted with LkCNR6. The interaction between LkCNR6 and LkHMP1, a protein associated with metal transport, was further confirmed by bimolecular fluorescence complementation (BiFC) assay. In summary, these results demonstrated that LkCNR6 plays important roles in positively responding zinc stress and negatively regulating organ development. These findings provide new insights into the regulatory mechanism of LkCNR6-mediated zinc stress response, and suggest the application of LkCNR6 in breeding high-heavy metal resistance crops.