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<p>Leaf explants of <i>Dracocephalum rupestre</i> were employed to investigate regeneration via direct differentiation pathways (DP) and indirect differentiation pathways (IDP). The DP medium for the leaf explants was Murashige and Skoog (MS) + 2.0 mg/L 6-Benzylaminopurine (BA) + 0.1 mg/L Kinetin (KIN) + 0.05 mg/L Naphthaleneacetic acid (NAA), achieving differentiation rate of 63.46%. The callus induction medium was MS + 2.0 mg/L BA + 0.1 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) + 0.5 mg/L Indole-3-acetic acid (IAA), achieving induction rate of 86.73%. For adventitious bud differentiation, the medium was MS + 2.0 mg/L BA + 2.0 mg/L Thidiazuron (TDZ) + 0.05 mg/L IAA, with a differentiation rate of 53.48%. In the DP, the adventitious bud proliferation medium is MS + 2.0 mg/L BA + 0.05 mg/L NAA, and the proliferation rate is 87.41%. In the IDP, the adventitious bud proliferation medium is MS + 2.0 mg/L BA + 0.01 mg/L NAA, with a proliferation rate of 82.96%. In the DP, the rooting medium for adventitious buds is ½MS + 0.5 mg/L NAA and rooting rate is 83.69%. For the IDP, the rooting medium for adventitious-bud is ½MS + 0.1 mg/L NAA + 0.1 mg/L IBA and rooting rate is 80.15%. Comparatively, the regeneration system of the DP is highly efficient and time-saving. The time from leaf explant being in vitro to regeneration is about 30 days less in the DP than in the IDP one. In this study, regeneration system for <i>D. rupestre</i> was successfully constructed through two different differentiation pathways. This system provides new approach for the efficient propagation of <i>D. rupestre</i>, facilitates large-scale seedling cultivation, and can provide stable resources for in-depth and systematic research on this species. Furthermore, it enables the full exploration and utilization of its unique commercial and medicinal values, contributing to the development of related industries.</p>

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Regeneration of in vitro plants through direct and indirect organogenesis from Dracocephalum rupestre leaf explants

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Jia li Wang,
  • Kang jie Yue,
  • Hui xin Liu,
  • Xu ping Tian

摘要

Leaf explants of Dracocephalum rupestre were employed to investigate regeneration via direct differentiation pathways (DP) and indirect differentiation pathways (IDP). The DP medium for the leaf explants was Murashige and Skoog (MS) + 2.0 mg/L 6-Benzylaminopurine (BA) + 0.1 mg/L Kinetin (KIN) + 0.05 mg/L Naphthaleneacetic acid (NAA), achieving differentiation rate of 63.46%. The callus induction medium was MS + 2.0 mg/L BA + 0.1 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) + 0.5 mg/L Indole-3-acetic acid (IAA), achieving induction rate of 86.73%. For adventitious bud differentiation, the medium was MS + 2.0 mg/L BA + 2.0 mg/L Thidiazuron (TDZ) + 0.05 mg/L IAA, with a differentiation rate of 53.48%. In the DP, the adventitious bud proliferation medium is MS + 2.0 mg/L BA + 0.05 mg/L NAA, and the proliferation rate is 87.41%. In the IDP, the adventitious bud proliferation medium is MS + 2.0 mg/L BA + 0.01 mg/L NAA, with a proliferation rate of 82.96%. In the DP, the rooting medium for adventitious buds is ½MS + 0.5 mg/L NAA and rooting rate is 83.69%. For the IDP, the rooting medium for adventitious-bud is ½MS + 0.1 mg/L NAA + 0.1 mg/L IBA and rooting rate is 80.15%. Comparatively, the regeneration system of the DP is highly efficient and time-saving. The time from leaf explant being in vitro to regeneration is about 30 days less in the DP than in the IDP one. In this study, regeneration system for D. rupestre was successfully constructed through two different differentiation pathways. This system provides new approach for the efficient propagation of D. rupestre, facilitates large-scale seedling cultivation, and can provide stable resources for in-depth and systematic research on this species. Furthermore, it enables the full exploration and utilization of its unique commercial and medicinal values, contributing to the development of related industries.