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<p>The medicinal herb <i>Linum album</i> is a rich source of lignans with various biological properties, especially anti-cancer activity. The present study was aimed to investigated the changes in the content of lignans i.e. podophyllotoxin (PTOX), 6-metoxypodophyllotoxin (6-MPTOX), and secoisolariciresinol (SECO) and the expression of their related genes in response to salt and osmotic stresses treatments including 28% (w v<sup>−1</sup>) PEG-6000 and 200 mM NaCl. Furthermore, the relationships between phytochemical responses and gene expressions under salt and osmotic stresses were investigated using previous transcriptomic data of <i>L. usitatissimum</i>, a model for lignans. Transcriptomic profiling showed that the gene expressions related to carbon metabolism, photosynthesis, and metabolic processes of nitrogen-containing compounds were significantly affected by these stresses. In addition, the specialized metabolites biosynthesis pathways changed significantly. In agreement with the transcriptomic results, the PTOX and 6-MPTOX contents were increased in the plantlet under these stresses, which showed a negative correlation with the expression of 5′-desmethyl-yatein O-methyltransferase (<i>OMT1</i>) and deoxyPTOX synthase (<i>2-ODD</i>) genes. The content of SECO, a substrate for SECO dehydrogenase (<i>SDH</i>), also indicated a significant negative correlation with the <i>SDH</i> gene expression. Results showed that salt and osmotic stresses could lead to an increase in the content of lignans through transcriptional regulations.</p>

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Gene expression and phytochemical responses of Linum album Kotschy ex Boiss. Cultured in vitro to salt and osmotic stresses

  • 期刊论文

  • 发表日期：

    2025年5月12日

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  • Zahra Danaeipour,
  • Ghasemali Garoosi,
  • Masoud Tohidfar,
  • Mohammad Reza Bakhtiarizadeh,
  • Mohammad Hossein Mirjalili

摘要

The medicinal herb Linum album is a rich source of lignans with various biological properties, especially anti-cancer activity. The present study was aimed to investigated the changes in the content of lignans i.e. podophyllotoxin (PTOX), 6-metoxypodophyllotoxin (6-MPTOX), and secoisolariciresinol (SECO) and the expression of their related genes in response to salt and osmotic stresses treatments including 28% (w v−1) PEG-6000 and 200 mM NaCl. Furthermore, the relationships between phytochemical responses and gene expressions under salt and osmotic stresses were investigated using previous transcriptomic data of L. usitatissimum, a model for lignans. Transcriptomic profiling showed that the gene expressions related to carbon metabolism, photosynthesis, and metabolic processes of nitrogen-containing compounds were significantly affected by these stresses. In addition, the specialized metabolites biosynthesis pathways changed significantly. In agreement with the transcriptomic results, the PTOX and 6-MPTOX contents were increased in the plantlet under these stresses, which showed a negative correlation with the expression of 5′-desmethyl-yatein O-methyltransferase (OMT1) and deoxyPTOX synthase (2-ODD) genes. The content of SECO, a substrate for SECO dehydrogenase (SDH), also indicated a significant negative correlation with the SDH gene expression. Results showed that salt and osmotic stresses could lead to an increase in the content of lignans through transcriptional regulations.