Scholarlink

<p>Gum Arabic (GA) significantly improves androgenesis from barley anthers. In this study, two-dimensional gel electrophoresis coupled to mass spectrometry was used to investigate the physiological mechanisms underlying GA-enhanced regeneration at the proteome level. The proteome of anthers after stress treatment, and those after 3 and 7 days of culture on induction medium (Im) and induction medium with 10&#xa0;mg L<sup>-1</sup> GA (GAm) was compared. Overall, 454 different protein spots were detected in the material studied, 61 spots were significantly changed in expression between the samples, and 44 proteins were successfully identified and classified into Gene Ontology categories. Principal Component Analysis revealed a much more homogeneous protein profile of anthers maintained on GAm than Im, indicative of a stabilization of cellular metabolism by GA. The proteomic differences between anthers cultured on GAm and Im were primarily quantitative, with 14 proteins showing at least a 1.5-fold increase and 2 proteins displaying a 5-fold increase in abundance on GAm. These proteins were involved in stress response (e.g. lactoylglutathione lyase), energy metabolism (e.g. ATP synthase subunit beta), cell division (actin-7), protein turnover (e.g. proteasome subunit beta type-6-like, leucine aminopeptidase 2), and nutrient storage (11&#xa0;S globulin seed storage protein). GA-induced qualitative proteome changes involved transient downregulation of only 4 proteins: luminal-binding protein 1 (two forms), glyceraldehyde 3-phosphate dehydrogenase and acidic form of fructokinase-2. Together, the proteomic analysis indicates that GA plays a complex role in regulating barley androgenesis helping to mitigate stress and thus create an environment that supports androgenic switch and effective energy management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of proteins associated with gum Arabic-enhancement of androgenesis from barley anthers

  • 期刊论文

  • 发表日期：

    2025年3月10日

查看全文
  • Robert Konieczny,
  • Tomasz A. Pawłowski,
  • Magdalena Surman,
  • Marek Šebela,
  • Magdalena Wilczak,
  • Kaja Skubała,
  • Sylwia Oleszczuk,
  • Janusz Zimny

摘要

Gum Arabic (GA) significantly improves androgenesis from barley anthers. In this study, two-dimensional gel electrophoresis coupled to mass spectrometry was used to investigate the physiological mechanisms underlying GA-enhanced regeneration at the proteome level. The proteome of anthers after stress treatment, and those after 3 and 7 days of culture on induction medium (Im) and induction medium with 10 mg L-1 GA (GAm) was compared. Overall, 454 different protein spots were detected in the material studied, 61 spots were significantly changed in expression between the samples, and 44 proteins were successfully identified and classified into Gene Ontology categories. Principal Component Analysis revealed a much more homogeneous protein profile of anthers maintained on GAm than Im, indicative of a stabilization of cellular metabolism by GA. The proteomic differences between anthers cultured on GAm and Im were primarily quantitative, with 14 proteins showing at least a 1.5-fold increase and 2 proteins displaying a 5-fold increase in abundance on GAm. These proteins were involved in stress response (e.g. lactoylglutathione lyase), energy metabolism (e.g. ATP synthase subunit beta), cell division (actin-7), protein turnover (e.g. proteasome subunit beta type-6-like, leucine aminopeptidase 2), and nutrient storage (11 S globulin seed storage protein). GA-induced qualitative proteome changes involved transient downregulation of only 4 proteins: luminal-binding protein 1 (two forms), glyceraldehyde 3-phosphate dehydrogenase and acidic form of fructokinase-2. Together, the proteomic analysis indicates that GA plays a complex role in regulating barley androgenesis helping to mitigate stress and thus create an environment that supports androgenic switch and effective energy management.