<p>In plant biotechnology, triploid breeding has become a useful method with substantial benefits for improving agriculture. Triplotypes, having three sets of chromosomes, frequently exhibit superior qualities such as greater resistance to environmental stressors, increased vigor, and seedlessness. This article explores the methods used to produce triploids, such as endosperm culture, somatic cell fusion, hybridization between diploid and tetraploid plants, and colchicine treatment. There are benefits and drawbacks to each technique, contributing to the diversity of triploid plants found in crop and horticultural species. The review also explores a wide range of applications of triploid plants, particularly in enhancing fruit quality, increasing biomass production, and improving stress tolerance. Examples from crops like citrus, bananas, and ornamental plants are highlighted to underscore the commercial and ecological significance of triploids. Additionally, we discuss over ways to detect and validate triploid plants in their early developmental phases by integrating molecular markers like single nucleotide polymorphisms (SNPs), amplified fragment length polymorphisms (AFLP), and simple sequence repeats (SSR). The effectiveness and accuracy of triploid breeding programs are greatly enhanced by these markers. This review also highlights the importance of integrating omics approaches, such as transcriptomics, proteomics, metabolomics, and genomics, in the study and development of triploid plant production which offers deeper insights into the genetic and molecular mechanisms underlying triploidy. In summary, the present study offers perspectives on the potential of triploid breeding in the future, highlighting its significance in sustainable agriculture and crop enhancement tactics.</p>