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<p><i>Maxillaria picta</i> is a Brazilian native orchid with a natural occurrence in the Atlantic Forest, a highly threatened and fragmented biodiversity hotspot. The in vitro culture is an excellent alternative for <i>ex-situ</i> orchid conservation and allows efficient plant propagation on a large scale. The 6-benzylaminopurine (BAP) is often used in in vitro culture to stimulate shoot formation. However, the morphoanatomical aspects of plantlets exposed to BAP and its residual effects remain unclear. Thus, we evaluated the morphoanatomical effects of BAP on in vitro culture and photosynthetic pigment concentration of <i>M. picta</i> acclimatized plants. Plantlets were obtained by in vitro seed germination and submitted to different concentrations of BAP, for 60 days. Roots and leaves were collected and morpho-anatomically evaluated. After 90 days of acclimatization, we quantified the photosynthetic pigments concentration of these plants. Our findings indicate that BAP supplementation significantly improves the formation of shoots and leaves, especially in treatment with 9 µM of BAP. However, concentrations above 9 µM of BAP increased tissue browning and significantly decreased the concentration of all photosynthetic pigments in acclimatized plants. Anatomically, 9 and 12 µM of BAP supplementation provided greater development of the vascular cylinder of the roots. Thus, this study provided new insights into the <i>M. picta in vitro</i> culture and showed the impact of BAP supplementation on the plantlets.</p>

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6-benzylaminopurine promotes the shoots formation during plantlets in vitro culture and affects the photosynthetic pigments accumulation in acclimatized plants of Maxillaria picta (Orchidaceae)

  • 期刊论文

  • 发表日期：

    2025年2月13日

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  • Fernanda Raach da Silva,
  • Clarissa Alves Stefanello,
  • Hugo Pacheco de Freitas Fraga

摘要

Maxillaria picta is a Brazilian native orchid with a natural occurrence in the Atlantic Forest, a highly threatened and fragmented biodiversity hotspot. The in vitro culture is an excellent alternative for ex-situ orchid conservation and allows efficient plant propagation on a large scale. The 6-benzylaminopurine (BAP) is often used in in vitro culture to stimulate shoot formation. However, the morphoanatomical aspects of plantlets exposed to BAP and its residual effects remain unclear. Thus, we evaluated the morphoanatomical effects of BAP on in vitro culture and photosynthetic pigment concentration of M. picta acclimatized plants. Plantlets were obtained by in vitro seed germination and submitted to different concentrations of BAP, for 60 days. Roots and leaves were collected and morpho-anatomically evaluated. After 90 days of acclimatization, we quantified the photosynthetic pigments concentration of these plants. Our findings indicate that BAP supplementation significantly improves the formation of shoots and leaves, especially in treatment with 9 µM of BAP. However, concentrations above 9 µM of BAP increased tissue browning and significantly decreased the concentration of all photosynthetic pigments in acclimatized plants. Anatomically, 9 and 12 µM of BAP supplementation provided greater development of the vascular cylinder of the roots. Thus, this study provided new insights into the M. picta in vitro culture and showed the impact of BAP supplementation on the plantlets.