Scholarlink

<p>Somatic embryogenesis is the preferred method for cell-to-plant regeneration of grapevines. In this study, we tested the embryogenic capacity of inflorescence-derived calli from 10 grape genotypes, representing a diverse group of&#xa0;<i>Vitis vinifera</i>&#xa0;and accessions of non-vinifera <i>Vitis</i> species. We describe the efficient and reproducible protocols for callus induction and regeneration in grape cultivars: “Aligote,” “Malbec,” “Saperavi,” “Carménère,” “Podarok Magaracha,” and rootstock “SO<sub>4</sub>.” Induction of callus formation was carried out in two stages. First, pro-embryogenic calli were obtained (1), and then these were transferred to medium for the induction of embryogenic calli formation (2). The resulting callus could be maintained in vitro on a liquid medium, or used for the somatic embryos initiation and regeneration of plants. The formation and the development of somatic embryos were carried out in three stages: (1) First, somatic embryos were initiated on a medium with a low content of auxin; (2) next, they were moved to a medium for somatic embryo maintenance; and (3) subsequently, only developed somatic embryos were transferred onto the medium for somatic embryo germination and development of shoots. For future biotechnological use of the studied cultivars, we established a stable transformation system for it on the basis of <i>Agrobacterium</i>-mediated transformation. The developed <i>Agrobacterium</i>-mediated transformation conditions yielded stably transformed lines of the Aligote and the Podarok Magaracha cultivars, confirmed by Southern blotting, with an efficiency of 0.36–0.43%.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Somatic embryogenesis and Agrobacterium-mediated transformation in a number of grape cultivars

  • 期刊论文

  • 发表日期：

    2025年3月4日

查看全文
  • Galina Maletich,
  • Igor Gavrilenko,
  • Alexander Pushin,
  • Svetlana Chelombit,
  • Tatyana Khmelnitskaya,
  • Yuri Plugatar,
  • Sergey Dolgov,
  • Pavel Khvatkov

摘要

Somatic embryogenesis is the preferred method for cell-to-plant regeneration of grapevines. In this study, we tested the embryogenic capacity of inflorescence-derived calli from 10 grape genotypes, representing a diverse group of Vitis vinifera and accessions of non-vinifera Vitis species. We describe the efficient and reproducible protocols for callus induction and regeneration in grape cultivars: “Aligote,” “Malbec,” “Saperavi,” “Carménère,” “Podarok Magaracha,” and rootstock “SO4.” Induction of callus formation was carried out in two stages. First, pro-embryogenic calli were obtained (1), and then these were transferred to medium for the induction of embryogenic calli formation (2). The resulting callus could be maintained in vitro on a liquid medium, or used for the somatic embryos initiation and regeneration of plants. The formation and the development of somatic embryos were carried out in three stages: (1) First, somatic embryos were initiated on a medium with a low content of auxin; (2) next, they were moved to a medium for somatic embryo maintenance; and (3) subsequently, only developed somatic embryos were transferred onto the medium for somatic embryo germination and development of shoots. For future biotechnological use of the studied cultivars, we established a stable transformation system for it on the basis of Agrobacterium-mediated transformation. The developed Agrobacterium-mediated transformation conditions yielded stably transformed lines of the Aligote and the Podarok Magaracha cultivars, confirmed by Southern blotting, with an efficiency of 0.36–0.43%.