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<p>The hairy root culture of <i>S. miltiorrhiza</i> offers a promising in vitro system for producing bioactive phenolic acids and tanshinones. However, the capacity for accumulating target metabolites requires further enhancement to meet industrial production demands. Auxin is known to regulate the biosynthesis of secondary metabolites in various in vitro plant cultures. To investigate whether auxin can act as an elicitor to enhance the levels of bioactive compounds in <i>S. miltiorrhiza</i> hairy roots, we treated the <i>S. miltiorrhiza</i> hairy roots with different concentrations of indole-3-acetic acid (IAA) and 1-naphthylacetic acid (NAA). The results indicated that both IAA and NAA at the selected concentrations had no obvious effect on the growth of <i>S. miltiorrhiza</i> hairy roots but significantly (<i>P</i> &lt; 0.05) stimulated the accumulation of phenolic acids and tanshinones, as well as up-regulated the expression of key enzyme genes in the corresponding biosynthetic pathways. Among the four treated groups, the application of 0.5 µM NAA most effectively promoted the accumulation of target compounds in this culture system and emerged as a strong elicitor candidate for industrial production. By integrating RNA-seq data and gene co-expression network analysis, we further identified genes in the auxin signaling pathway and transcription factors that may be involved in the auxin-induced accumulation of phenolic acids and tanshinones in <i>S. miltiorrhiza</i> hairy roots. Our findings elucidate the role of auxin as a stimulator in the biosynthesis of phenolic acids and tanshinones in <i>S. miltiorrhiza</i> hairy roots and provide candidate genes for further investigation of the underlying molecular mechanisms.</p>

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Auxin promotes the production of phenolic acids and tanshinones in Salvia miltiorrhiza hairy roots via auxin signaling and transcriptional pathways

  • 期刊论文

  • 发表日期：

    2025年1月30日

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  • Shuncang Zhang,
  • Yuanyuan Gao,
  • Wei Wang,
  • Lin Qiu,
  • Ziyang Zhao,
  • Xiaoyu Liu,
  • Ning Yang,
  • Dongfeng Yang

摘要

The hairy root culture of S. miltiorrhiza offers a promising in vitro system for producing bioactive phenolic acids and tanshinones. However, the capacity for accumulating target metabolites requires further enhancement to meet industrial production demands. Auxin is known to regulate the biosynthesis of secondary metabolites in various in vitro plant cultures. To investigate whether auxin can act as an elicitor to enhance the levels of bioactive compounds in S. miltiorrhiza hairy roots, we treated the S. miltiorrhiza hairy roots with different concentrations of indole-3-acetic acid (IAA) and 1-naphthylacetic acid (NAA). The results indicated that both IAA and NAA at the selected concentrations had no obvious effect on the growth of S. miltiorrhiza hairy roots but significantly (P < 0.05) stimulated the accumulation of phenolic acids and tanshinones, as well as up-regulated the expression of key enzyme genes in the corresponding biosynthetic pathways. Among the four treated groups, the application of 0.5 µM NAA most effectively promoted the accumulation of target compounds in this culture system and emerged as a strong elicitor candidate for industrial production. By integrating RNA-seq data and gene co-expression network analysis, we further identified genes in the auxin signaling pathway and transcription factors that may be involved in the auxin-induced accumulation of phenolic acids and tanshinones in S. miltiorrhiza hairy roots. Our findings elucidate the role of auxin as a stimulator in the biosynthesis of phenolic acids and tanshinones in S. miltiorrhiza hairy roots and provide candidate genes for further investigation of the underlying molecular mechanisms.