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<p><i>Spathiphyllum</i> species are highly valued ornamental plants, renowned for their exceptional air-purifying properties and commonly propagated through tissue culture. However, gaps in existing research on rooting and acclimatization restrict the optimization of propagation protocols. This study provides the first in-depth analysis of how explant size and naphthaleneacetic acid (NAA) concentration influence rooting efficiency and how explant size interacts with substrate type during the acclimatization phase of <i>Spathiphyllum wallisii</i>. Rooting efficiency was significantly affected by explant size and NAA concentration. Larger explants (2–3 cm) exhibited a substantial increase in root number, from 4.07 roots per explant without NAA to 23.83 roots with 1 mg L⁻<sup>1</sup> NAA, representing a sixfold improvement. Medium-sized explants (1–2 cm) showed moderate rooting responses, increasing from 5.67 to 8.52 roots per explant. In contrast, smaller explants (0.5–1 cm) demonstrated limited rooting success with reduced response to NAA. During acclimatization, smaller vitroplants (1–2 cm) achieved a maximum height of only 4.9 cm, while larger vitroplants (3–4 cm) demonstrated superior development, reaching an average height of 12.32 cm in a 1:1 peat moss and fiber substrate. This substrate composition also facilitated robust shoot and root development, yielding a maximum average of 45 roots per plantlet and 97% of plantlets developing new shoots. These findings highlights the importance of optimizing explant size and NAA concentration to enhance both rooting efficiency and subsequent in vivo growth, thereby refining tissue culture methodologies for this valuable ornamental species.</p>

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Enhancing in vitro rooting and acclimatization of Spathiphyllum wallisii through explant size, NAA, and substrate selection

  • 期刊论文

  • 发表日期：

    2025年1月31日

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  • Imane Boudadi,
  • Soumaya El Merzougui,
  • Khadija Lachguer,
  • Khalid Lagram,
  • Rachida El boullani,
  • Mohammed Amine Serghini

摘要

Spathiphyllum species are highly valued ornamental plants, renowned for their exceptional air-purifying properties and commonly propagated through tissue culture. However, gaps in existing research on rooting and acclimatization restrict the optimization of propagation protocols. This study provides the first in-depth analysis of how explant size and naphthaleneacetic acid (NAA) concentration influence rooting efficiency and how explant size interacts with substrate type during the acclimatization phase of Spathiphyllum wallisii. Rooting efficiency was significantly affected by explant size and NAA concentration. Larger explants (2–3 cm) exhibited a substantial increase in root number, from 4.07 roots per explant without NAA to 23.83 roots with 1 mg L⁻1 NAA, representing a sixfold improvement. Medium-sized explants (1–2 cm) showed moderate rooting responses, increasing from 5.67 to 8.52 roots per explant. In contrast, smaller explants (0.5–1 cm) demonstrated limited rooting success with reduced response to NAA. During acclimatization, smaller vitroplants (1–2 cm) achieved a maximum height of only 4.9 cm, while larger vitroplants (3–4 cm) demonstrated superior development, reaching an average height of 12.32 cm in a 1:1 peat moss and fiber substrate. This substrate composition also facilitated robust shoot and root development, yielding a maximum average of 45 roots per plantlet and 97% of plantlets developing new shoots. These findings highlights the importance of optimizing explant size and NAA concentration to enhance both rooting efficiency and subsequent in vivo growth, thereby refining tissue culture methodologies for this valuable ornamental species.