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<p>Seeds represent the continuation of plant life, but as the influence of genetic properties of seeds and environmental factors in vitro, there is usually an irreversible decline in viability. Therefore, effective preservation methods are very important for long-term seed preservation, especially for rare and endangered plants. In this study, we used the seeds of the rare and endangered plant <i>Elaeagnus mollis</i> as materials to compare and analyze the changes of seed viability and apoptosis-like programmed cell death under different preservation methods, to explore the effects of preservation methods on seed viability and the role of apoptosis-like programmed cell death in seed viability changes. The results showed that mesocarp seeds significantly alleviated viability decline within 1 month of storage, while exocarp seeds had a relatively stable viability change during 5 months, and the viability decline was alleviated when stored at -20℃, -40℃, -80℃ and − 196℃. And with the seed viability decreases, all seeds of <i>E. mollis</i> stored under different methods underwent apoptosis-like programmed cell death, which mainly manifested as decreased mitochondrial membrane potential, increased number of apoptotic cells and necrotic cells, activated caspase-3-like and caspase-9-like proteases, and gradually increased production of apoptosis-like programmed cell death signaling molecule nitric oxide (NO). Correlation analysis further showed that seed viability changes under different preservation conditions were positively correlated with apoptosis-like programmed cell death index mitochondrial membrane potential, and negatively correlated with caspase-3-like, caspase-9-like protease and apoptosis-like programmed cell death signaling molecule NO. These results indicated that apoptosis-like programmed cell death was one of the reasons for <i>E. mollis</i> seed viability decrease. At 4℃, the mitochondrial membrane potential decreased more seriously, which promoted the release of more signaling molecules and significantly enhanced the downstream caspase protease activity. Moreover, preserving exocarp seeds below − 20℃ could alleviate this process and maintain seed viability.</p>

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Screening of optimal preservation methods of Elaeagnus mollis seeds and the role of apoptosis-like programmed cell death in seed viability

  • 期刊论文

  • 发表日期：

    2025年2月7日

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  • Guo Jiayi,
  • Zhu Qinlu,
  • Huang Jiaqi,
  • Wang Zhiling,
  • Ren Ruifen

摘要

Seeds represent the continuation of plant life, but as the influence of genetic properties of seeds and environmental factors in vitro, there is usually an irreversible decline in viability. Therefore, effective preservation methods are very important for long-term seed preservation, especially for rare and endangered plants. In this study, we used the seeds of the rare and endangered plant Elaeagnus mollis as materials to compare and analyze the changes of seed viability and apoptosis-like programmed cell death under different preservation methods, to explore the effects of preservation methods on seed viability and the role of apoptosis-like programmed cell death in seed viability changes. The results showed that mesocarp seeds significantly alleviated viability decline within 1 month of storage, while exocarp seeds had a relatively stable viability change during 5 months, and the viability decline was alleviated when stored at -20℃, -40℃, -80℃ and − 196℃. And with the seed viability decreases, all seeds of E. mollis stored under different methods underwent apoptosis-like programmed cell death, which mainly manifested as decreased mitochondrial membrane potential, increased number of apoptotic cells and necrotic cells, activated caspase-3-like and caspase-9-like proteases, and gradually increased production of apoptosis-like programmed cell death signaling molecule nitric oxide (NO). Correlation analysis further showed that seed viability changes under different preservation conditions were positively correlated with apoptosis-like programmed cell death index mitochondrial membrane potential, and negatively correlated with caspase-3-like, caspase-9-like protease and apoptosis-like programmed cell death signaling molecule NO. These results indicated that apoptosis-like programmed cell death was one of the reasons for E. mollis seed viability decrease. At 4℃, the mitochondrial membrane potential decreased more seriously, which promoted the release of more signaling molecules and significantly enhanced the downstream caspase protease activity. Moreover, preserving exocarp seeds below − 20℃ could alleviate this process and maintain seed viability.