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<p>The current investigation successfully established the competent role of Putrescine in the clonal propagation of <i>Nothapodytes foetida.</i> Various concentrations of plant growth regulators alone and in combination with polyamines were employed in the Murashige and Skoog’s medium to evaluate their influence on the regeneration capacity of the cotyledonary node, hypocotyl, and leaf explants. Among these, Thidiazuron (1 ppm), Meta-Topolin (1.5 ppm), and 6-Benzylaminopurine (2 ppm) along with Indole acetic acid (0.1ppm) were effective in inducing the optimum number of shoots (25.89 ± 0.72, 18.22 ± 0.87 and 14.2 ± 0.68 respectively) from the leaf explant. The effect of Polyamines on shoot proliferation was studied by supplementing Putrescine, and Spermidine (50–200µM) in the regeneration medium. The inclusion of Putrescine in the regeneration medium has significantly increased the shoot proliferation two-fold (52.00 ± 0.32), as observed in the medium enriched with Thidiazuron (1ppm) and Putrescine (100µM). In contrast, Spermidine exhibited no notable influence on shoot multiplication. The propagated plantlets (88%) were rooted on a ½ MS medium amended with Indole-3-butyric acid (1.5 ppm). 80% of the regenerated plantlets survived during acclimatization in greenhouse conditions. Further, genetic fidelity was assessed employing two markers, ISSR and SCoT with monomorphic banding patterns of regenerants, indicating no somaclonal variations and their resemblance to the mother plant. In this regard, this is the first report to assess the role of Putrescine, a polyamine in the regeneration potential of <i>Nothapodytes foetida</i> with SCoT markers to evaluate genetic fidelity. Results indicate that this is an efficient and rapid protocol with Putrescine as a positive modulator for in vitro propagation of <i>Nothapodytes foetida.</i></p> Graphical Abstract <p></p>

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The synergistic effect of plant growth regulators and polyamines on regeneration of Nothapodytes foetida, and assessment of genetic fidelity using ISSR and SCoT markers

  • 期刊论文

  • 发表日期：

    2025年2月3日

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  • Srividya Attaluri,
  • Rathnaprabha Dharavath

摘要

The current investigation successfully established the competent role of Putrescine in the clonal propagation of Nothapodytes foetida. Various concentrations of plant growth regulators alone and in combination with polyamines were employed in the Murashige and Skoog’s medium to evaluate their influence on the regeneration capacity of the cotyledonary node, hypocotyl, and leaf explants. Among these, Thidiazuron (1 ppm), Meta-Topolin (1.5 ppm), and 6-Benzylaminopurine (2 ppm) along with Indole acetic acid (0.1ppm) were effective in inducing the optimum number of shoots (25.89 ± 0.72, 18.22 ± 0.87 and 14.2 ± 0.68 respectively) from the leaf explant. The effect of Polyamines on shoot proliferation was studied by supplementing Putrescine, and Spermidine (50–200µM) in the regeneration medium. The inclusion of Putrescine in the regeneration medium has significantly increased the shoot proliferation two-fold (52.00 ± 0.32), as observed in the medium enriched with Thidiazuron (1ppm) and Putrescine (100µM). In contrast, Spermidine exhibited no notable influence on shoot multiplication. The propagated plantlets (88%) were rooted on a ½ MS medium amended with Indole-3-butyric acid (1.5 ppm). 80% of the regenerated plantlets survived during acclimatization in greenhouse conditions. Further, genetic fidelity was assessed employing two markers, ISSR and SCoT with monomorphic banding patterns of regenerants, indicating no somaclonal variations and their resemblance to the mother plant. In this regard, this is the first report to assess the role of Putrescine, a polyamine in the regeneration potential of Nothapodytes foetida with SCoT markers to evaluate genetic fidelity. Results indicate that this is an efficient and rapid protocol with Putrescine as a positive modulator for in vitro propagation of Nothapodytes foetida.

Graphical Abstract