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<p>Chilling is a type of abiotic stress that limits plant distribution and production. To improve plant chilling resistance, the chilling response gene <i>ZsDRT102</i> was cloned from <i>Zelkova schneideriana</i> Hand.-Mazz. This gene is 942&#xa0;bp long and encodes a protein related to DNA damage repair (DDR). Expression analysis of <i>ZsDRT102</i> showed a 3.6-fold higher level in the chilling-resistant lines compared to the chilling-sensitive lines. Tobacco plants with <i>ZsDRT102</i> overexpression significantly improved the morphological performance under chilling stress compared with wild-type (WT) plants. Under chilling stress, physiological index assays showed that the malondialdehyde (MDA) content was 10.3–26.2% lower, relative electrical leakage (REL) value was 24.5–35.3% lower, and chlorophyll content was 1.41–1.54-fold higher in transgenic plants than in WT plants. The results suggested that <i>ZsDRT102</i> is an effective gene resource in molecular breeding programs against low-temperature stress, providing new insights into defense against abiotic stress through DDR.</p>

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Overexpression of ZsDRT102 from Zelkova schneideriana increases the chilling resistance of plants

  • 期刊论文

  • 发表日期：

    2025年1月2日

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  • Lei Wang,
  • Xiao Liu,
  • Longfeng Gong,
  • Jiayu Hu,
  • Xiaoxiong Lu,
  • Jichen Xu

摘要

Chilling is a type of abiotic stress that limits plant distribution and production. To improve plant chilling resistance, the chilling response gene ZsDRT102 was cloned from Zelkova schneideriana Hand.-Mazz. This gene is 942 bp long and encodes a protein related to DNA damage repair (DDR). Expression analysis of ZsDRT102 showed a 3.6-fold higher level in the chilling-resistant lines compared to the chilling-sensitive lines. Tobacco plants with ZsDRT102 overexpression significantly improved the morphological performance under chilling stress compared with wild-type (WT) plants. Under chilling stress, physiological index assays showed that the malondialdehyde (MDA) content was 10.3–26.2% lower, relative electrical leakage (REL) value was 24.5–35.3% lower, and chlorophyll content was 1.41–1.54-fold higher in transgenic plants than in WT plants. The results suggested that ZsDRT102 is an effective gene resource in molecular breeding programs against low-temperature stress, providing new insights into defense against abiotic stress through DDR.